A compact microscope for voltage imaging

X. Meng; L. Huismans; T. Huijben; G. Szabo; R. Van Tol; I. De Heer; S. Ganapathy; D. Brinks

doi:10.1088/2040-8986/ac5dd5

A compact microscope for voltage imaging

X. Meng, L. Huismans, T. Huijben, G. Szabo, R. Van Tol, I. De Heer, S. Ganapathy, D. Brinks^*

^*Corresponding author for this work

Molecular Genetics

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

Voltage imaging and optogenetics offer new routes to optically detect and influence neural dynamics. Optimized hardware is necessary to make the most of these new techniques. Here we present the Octoscope, a versatile, multimodal device for all-optical electrophysiology. We illustrate its concept and design and demonstrate its capability to perform both 1-photon and 2-photon voltage imaging with spatial and temporal light patterning, in both inverted and upright configurations, in vitro and in vivo.

Original language	English
Article number	054004
Journal	Journal of Optics (United Kingdom)
Volume	24
Issue number	5
DOIs	https://doi.org/10.1088/2040-8986/ac5dd5
Publication status	Published - May 2022

Bibliographical note

Funding Information:
We thank Adam Cohen for the gift of QuasAr1 (derived from Addgene plasmid # 59172), Ed Boyden for the gift of Archon1 (Addgene plasmid # 108423), and Elizabeth Carroll, Laura Maddalena and Zhenzhen Wu for use of zebrafish larvae. DB acknowledges support by an NWO Start-up Grant (740.018.018) and ERC Starting Grant (850818—MULTIVIsion).

Publisher Copyright:
© 2022 The Author(s). Published by IOP Publishing Ltd.

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10.1088/2040-8986/ac5dd5Licence: CC BY

Meng_2022_J._Opt._24_054004Final published version, 2.22 MBLicence: CC BY

https://www.scopus.com/inward/record.uri?eid=2-s2.0-85128369245&doi=10.1088%2f2040-8986%2fac5dd5&partnerID=40&md5=96d7dd3550e7fa2050ff074a47d56948

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abstract = "Voltage imaging and optogenetics offer new routes to optically detect and influence neural dynamics. Optimized hardware is necessary to make the most of these new techniques. Here we present the Octoscope, a versatile, multimodal device for all-optical electrophysiology. We illustrate its concept and design and demonstrate its capability to perform both 1-photon and 2-photon voltage imaging with spatial and temporal light patterning, in both inverted and upright configurations, in vitro and in vivo.",

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AU - Meng, X.

AU - Huismans, L.

AU - Huijben, T.

AU - Szabo, G.

AU - Van Tol, R.

AU - De Heer, I.

AU - Ganapathy, S.

AU - Brinks, D.

N1 - Funding Information: We thank Adam Cohen for the gift of QuasAr1 (derived from Addgene plasmid # 59172), Ed Boyden for the gift of Archon1 (Addgene plasmid # 108423), and Elizabeth Carroll, Laura Maddalena and Zhenzhen Wu for use of zebrafish larvae. DB acknowledges support by an NWO Start-up Grant (740.018.018) and ERC Starting Grant (850818—MULTIVIsion). Publisher Copyright: © 2022 The Author(s). Published by IOP Publishing Ltd.

PY - 2022/5

Y1 - 2022/5

N2 - Voltage imaging and optogenetics offer new routes to optically detect and influence neural dynamics. Optimized hardware is necessary to make the most of these new techniques. Here we present the Octoscope, a versatile, multimodal device for all-optical electrophysiology. We illustrate its concept and design and demonstrate its capability to perform both 1-photon and 2-photon voltage imaging with spatial and temporal light patterning, in both inverted and upright configurations, in vitro and in vivo.

AB - Voltage imaging and optogenetics offer new routes to optically detect and influence neural dynamics. Optimized hardware is necessary to make the most of these new techniques. Here we present the Octoscope, a versatile, multimodal device for all-optical electrophysiology. We illustrate its concept and design and demonstrate its capability to perform both 1-photon and 2-photon voltage imaging with spatial and temporal light patterning, in both inverted and upright configurations, in vitro and in vivo.

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DO - 10.1088/2040-8986/ac5dd5

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JO - Journal of Optics (United Kingdom)

JF - Journal of Optics (United Kingdom)

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A compact microscope for voltage imaging

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