A culture model to analyze the acute biomaterial-dependent reaction of human primary neutrophils in vitro

Marinus A. Wesdorp; Andrea Schwab; Ezgi Irem Bektas; Roberto Narcisi; David Eglin; Martin J. Stoddart; Gerjo J.V.M. Van Osch; Matteo D'Este

doi:10.1016/j.bioactmat.2022.05.036

A culture model to analyze the acute biomaterial-dependent reaction of human primary neutrophils in vitro

Marinus A. Wesdorp, Andrea Schwab, Ezgi Irem Bektas, Roberto Narcisi, David Eglin, Martin J. Stoddart, Gerjo J.V.M. Van Osch, Matteo D'Este^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

Neutrophils play a pivotal role in orchestrating the immune system response to biomaterials, the onset and resolution of chronic inflammation, and macrophage polarization. However, the neutrophil response to biomaterials and the consequent impact on tissue engineering approaches is still scarcely understood. Here, we report an in vitro culture model that comprehensively describes the most important neutrophil functions in the light of tissue repair. We isolated human primary neutrophils from peripheral blood and exposed them to a panel of hard, soft, naturally- and synthetically-derived materials. The overall trend showed increased neutrophil survival on naturally derived constructs, together with higher oxidative burst, decreased myeloperoxidase and neutrophil elastase and decreased cytokine secretion compared to neutrophils on synthetic materials. The culture model is a step to better understand the immune modulation elicited by biomaterials. Further studies are needed to correlate the neutrophil response to tissue healing and to elucidate the mechanism triggering the cell response and their consequences in determining inflammation onset and resolution.

Original language	English
Pages (from-to)	627-637
Number of pages	11
Journal	Bioactive Materials
Volume	20
DOIs	https://doi.org/10.1016/j.bioactmat.2022.05.036
Publication status	Published - Feb 2023

Bibliographical note

Acknowledgements
The authors express thanks to Dr. Milena Sokolowska, Swiss Institute of Allergy and Asthma Research, for valuable input and to introduce us to the Olink® technique, and to Dr. Anja Heider from the same institution for support with the Olink® analysis, and Dr Claire Duchez, INSERM, U1059 SAINBIOSE for valuable feedback. This work received financial support from the AO Foundation through the Osteochondral Defect Collaborative Research Program and through an AO CMF grant. We would like to thank all volunteers who donated their blood.

Publisher Copyright: © 2022 The Authors

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1-s2.0-S2452199X22002584-mainFinal published version, 5.11 MBLicence: CC BY-NC-ND

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title = "A culture model to analyze the acute biomaterial-dependent reaction of human primary neutrophils in vitro",

abstract = "Neutrophils play a pivotal role in orchestrating the immune system response to biomaterials, the onset and resolution of chronic inflammation, and macrophage polarization. However, the neutrophil response to biomaterials and the consequent impact on tissue engineering approaches is still scarcely understood. Here, we report an in vitro culture model that comprehensively describes the most important neutrophil functions in the light of tissue repair. We isolated human primary neutrophils from peripheral blood and exposed them to a panel of hard, soft, naturally- and synthetically-derived materials. The overall trend showed increased neutrophil survival on naturally derived constructs, together with higher oxidative burst, decreased myeloperoxidase and neutrophil elastase and decreased cytokine secretion compared to neutrophils on synthetic materials. The culture model is a step to better understand the immune modulation elicited by biomaterials. Further studies are needed to correlate the neutrophil response to tissue healing and to elucidate the mechanism triggering the cell response and their consequences in determining inflammation onset and resolution.",

author = "Wesdorp, \{Marinus A.\} and Andrea Schwab and Bektas, \{Ezgi Irem\} and Roberto Narcisi and David Eglin and Stoddart, \{Martin J.\} and \{Van Osch\}, \{Gerjo J.V.M.\} and Matteo D'Este",

note = "Acknowledgements The authors express thanks to Dr. Milena Sokolowska, Swiss Institute of Allergy and Asthma Research, for valuable input and to introduce us to the Olink{\textregistered} technique, and to Dr. Anja Heider from the same institution for support with the Olink{\textregistered} analysis, and Dr Claire Duchez, INSERM, U1059 SAINBIOSE for valuable feedback. This work received financial support from the AO Foundation through the Osteochondral Defect Collaborative Research Program and through an AO CMF grant. We would like to thank all volunteers who donated their blood. Publisher Copyright: {\textcopyright} 2022 The Authors",

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doi = "10.1016/j.bioactmat.2022.05.036",

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AU - Schwab, Andrea

AU - Bektas, Ezgi Irem

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AU - Eglin, David

AU - Stoddart, Martin J.

AU - Van Osch, Gerjo J.V.M.

AU - D'Este, Matteo

N1 - Acknowledgements The authors express thanks to Dr. Milena Sokolowska, Swiss Institute of Allergy and Asthma Research, for valuable input and to introduce us to the Olink® technique, and to Dr. Anja Heider from the same institution for support with the Olink® analysis, and Dr Claire Duchez, INSERM, U1059 SAINBIOSE for valuable feedback. This work received financial support from the AO Foundation through the Osteochondral Defect Collaborative Research Program and through an AO CMF grant. We would like to thank all volunteers who donated their blood. Publisher Copyright: © 2022 The Authors

PY - 2023/2

Y1 - 2023/2

N2 - Neutrophils play a pivotal role in orchestrating the immune system response to biomaterials, the onset and resolution of chronic inflammation, and macrophage polarization. However, the neutrophil response to biomaterials and the consequent impact on tissue engineering approaches is still scarcely understood. Here, we report an in vitro culture model that comprehensively describes the most important neutrophil functions in the light of tissue repair. We isolated human primary neutrophils from peripheral blood and exposed them to a panel of hard, soft, naturally- and synthetically-derived materials. The overall trend showed increased neutrophil survival on naturally derived constructs, together with higher oxidative burst, decreased myeloperoxidase and neutrophil elastase and decreased cytokine secretion compared to neutrophils on synthetic materials. The culture model is a step to better understand the immune modulation elicited by biomaterials. Further studies are needed to correlate the neutrophil response to tissue healing and to elucidate the mechanism triggering the cell response and their consequences in determining inflammation onset and resolution.

AB - Neutrophils play a pivotal role in orchestrating the immune system response to biomaterials, the onset and resolution of chronic inflammation, and macrophage polarization. However, the neutrophil response to biomaterials and the consequent impact on tissue engineering approaches is still scarcely understood. Here, we report an in vitro culture model that comprehensively describes the most important neutrophil functions in the light of tissue repair. We isolated human primary neutrophils from peripheral blood and exposed them to a panel of hard, soft, naturally- and synthetically-derived materials. The overall trend showed increased neutrophil survival on naturally derived constructs, together with higher oxidative burst, decreased myeloperoxidase and neutrophil elastase and decreased cytokine secretion compared to neutrophils on synthetic materials. The culture model is a step to better understand the immune modulation elicited by biomaterials. Further studies are needed to correlate the neutrophil response to tissue healing and to elucidate the mechanism triggering the cell response and their consequences in determining inflammation onset and resolution.

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A culture model to analyze the acute biomaterial-dependent reaction of human primary neutrophils in vitro

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