Abstract
Background: The present study aimed to explore the correlation between calcium-acti-vated potassium channels, left atrial flow field mechanics, valvular atrial fibrillation (VAF), and thrombosis. The process of transforming mechanical signals into biological signals has been revealed, which offers new insights into the study of VAF. Methods: Computational fluid dynamics simulations use numeric analysis and algorithms to compute flow parameters, including turbulent shear stress (TSS) and wall pressure in the left atrium (LA). Real-time PCR and western blotting were used to detect the mRNA and protein expression of IKCa2.3/3.1, ATK1, and P300 in the left atrial tissue of 90 patients. Results: In the valvular disease group, the TSS and wall ressure in the LA increased, the wall pressure increased in turn in all disease groups, mainly near the mitral valve and the posterior portion of the LA, the increase in TSS was the most significant in each group near the mitral valve, and the middle and lower part of the back of the LA and the mRNA expression and protein expression levels of IKCa2.3/3.1, AKT1, and P300 increased (p < 0.05) (n = 15). The present study was preliminarily conducted to elucidate whether there might be a certain correlation between IKCa2.3 and LA hemodynamic changes. Conclusions: The TSS and wall pressure changes in the LA are correlated with the upregulation of mRNA and protein expression of IKCa2.3/3.1, AKT1, and P300.
Original language | English |
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Article number | 1383 |
Journal | Cells |
Volume | 11 |
Issue number | 9 |
DOIs | |
Publication status | Published - 19 Apr 2022 |
Bibliographical note
Funding Information:Author Contributions: The authors declare that all data were generated in-house and that no paper mill was used. Y.S. (Yi Sun) and H.Z. conceived and designed the project; X.P., J.L., Y.S. (Yi Sun) and L.D. subject to guide. P.S. and Y.S. (Yi Sun) completed the experiment. Y.S. (Yi Sun) acquisition of the financial support. H.Z. and G.L. hydrodynamic modeling. Z.L. ultrasound. Y.S. (Yi Song) extracorporeal circulation during surgery. J.X. MIMICS drawing. E.Y. and X.C. numerical simulation. M.L. CAD modeling. S.J. image analysis. G.Z., E.Y., J.L., R.M., Y.S. (Yi Song), J.X., M.L., S.J., X.W. and X.C., acquired the data; X.P., L.K., P.S., M.F., Z.L. and G.L. analyzed and interpreted the data; and Y.S. (Yi Sun), P.S., M.F., L.D. and G.L. wrote the paper. M.F., L.K., J.L. and L.D. grammar check. R.M., M.F., L.K., Y.S. (Yi Song) and X.W. revised the manuscript, R.M., X.W., X.P. and G.Z. completed the operation and took a specimen. All authors have read and agreed to the published version of the manuscript and are accountable for ensuring all aspects of the work.
Funding:
This study was supported by the Medical Reserve Talented Person of Yunnan Provincial
Health and Family Planning Commission (Grant Nos. H-2017017 and H-2017015), the Association
Foundation Program of Yunnan Provincial Science and Technology Department and Kunming
Medical University (Grant Nos. 2018FE001(-076) and 2018 FE001(-106)), and the National Science
Foundation of China (Grant No. 81960072).
Publisher Copyright:
© 2022 by the authors. Licensee MDPI, Basel, Switzerland.