A DNA vaccine coding for glycoprotein B of pseudorabies virus induces cell-mediated immunity in pigs and reduces virus excretion early after infection

E. M.A. Van Rooij*, B. L. Haagmans, H. L. Glansbeek, Y. E. De Visser, M. G.M. De Bruin, W. Boersma, A. T.J. Bianchi

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

40 Citations (Scopus)

Abstract

Glycoproteins B (gB), gC and gD of pseudorabies virus (PRV) have been implicated as important antigens in protective immunity against PRV infection. As cell-mediated immunity plays a major role in this protective immunity, we determined the significance of these glycoproteins in the actual induction of cell-mediated immunity. We vaccinated pigs with plasmid DNA constructs coding for gB, gC or gD and challenged them with the virulent NIA-3 strain of pseudorabies virus. Vaccination with plasmid DNA coding for gB induced the strongest cell-mediated immune responses including cytotoxic T cell responses, whereas plasmid DNA coding for gD induced the strongest virus neutralising antibody responses. Interestingly, vaccination with gB-DNA reduced virus excretion early after challenge infection while vaccination with gC-DNA or gD-DNA did not.This is the first study to demonstrate that DNA vaccination induces cytotoxic T cell responses in pigs and that cell-mediated immunity induced by vaccination with gB-DNA is important for the reduction of virus excretion early after challenge infection. 

Original languageEnglish
Pages (from-to)121-136
Number of pages16
JournalVeterinary Immunology and Immunopathology
Volume74
Issue number1-2
Early online date7 Apr 2000
DOIs
Publication statusPublished - 19 Apr 2000
Externally publishedYes

Bibliographical note

Copyright © 2000 Elsevier Science B.V.

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