A genome-wide linkage scan in a Dutch family identifies a premature ovarian failure susceptibility locus

Rogier Oldenburg, Marieke van Dooren, Bianca de Graaf, Erik Simons, LCP Govaerts, Sigrid Swagemakers, Annemieke Verkerk, Ben Oostra, AM Bertoli Avella

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31 Citations (Scopus)

Abstract

Premature ovarian failure (POF) is characterized by elevated gonadotrophins and amenorrhea before the age of 40 years and occurs approximately in 1% of women. POF etiology is highly heterogeneous with a wide spectrum of etiological pathogenic mechanisms including genetic causes. These mostly involve numerical, structural or monogenic defects on the X-chromosome. Mutations in a small number of autosomal genes (such as FOXL2 and NOBOX) have been identified as a cause of POF. However, in most cases, the disease underlying mechanisms are largely unknown. We performed a genome-wide linkage analysis in a relatively large Dutch family with seven patients suffering from POF, showing a dominant pattern of inheritance. A genome-wide analysis, using 50K single nucleotide polymorphism arrays, was combined with conventional parametric linkage analysis. We identified three genomic regions on chromosomes 5, 14 and 18 yielding suggestive linkage (multipoint LOD score of 2.4 for each region). After inclusion of one elder unaffected family member, only the region on chromosome 5 remains as a putative POF locus. In addition, we investigated a second family (three living patients over three generations) for the regions on chromosome 5, 14 and 18. Haplotype analysis supported only the locus on chromosome 5q14.1-q15. We performed the first genome-wide linkage search in familial POF and identified a region on chromosome 5q14.1-q15, which may harbor a novel POF susceptibility gene.
Original languageUndefined/Unknown
Pages (from-to)2835-2841
Number of pages7
JournalHuman Reproduction
Volume23
Issue number12
DOIs
Publication statusPublished - 2008

Research programs

  • EMC MGC-02-02-01
  • EMC MGC-02-96-01

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