A high-density integrated genetic linkage and radiation hybrid map of the laboratory rat

Robert G. Steen; Anne E. Kwitek-Black; Christopher Glenn; Jo Gullings-Handley; William Van Etten; O. Scott Atkinson; Diane Appel; Simon Twigger; Melanie Muir; Tim Mull; Mary Granados; Mushira Kissebah; Kerri Russo; Robbin Crane; Michael Popp; Marc Peden; Tara Matise; Donna M. Brown; Jian Lu; Stephen Kingsmore; Peter J. Tonellato; Steve Rozen; Donna Slonim; Peter Young; Margit Knoblauch; Abraham Provoost; Detlev Ganten; Steven D. Colman; Jonathan Rothberg; Eric S. Lander; Howard J. Jacob

doi:10.1101/gr.9.6.AP1

A high-density integrated genetic linkage and radiation hybrid map of the laboratory rat

Robert G. Steen, Anne E. Kwitek-Black, Christopher Glenn, Jo Gullings-Handley, William Van Etten, O. Scott Atkinson, Diane Appel, Simon Twigger, Melanie Muir, Tim Mull, Mary Granados, Mushira Kissebah, Kerri Russo, Robbin Crane, Michael Popp, Marc Peden, Tara Matise, Donna M. Brown, Jian Lu, Stephen KingsmorePeter J. Tonellato, Steve Rozen, Donna Slonim, Peter Young, Margit Knoblauch, Abraham Provoost, Detlev Ganten, Steven D. Colman, Jonathan Rothberg, Eric S. Lander, Howard J. Jacob^*

^*Corresponding author for this work

Pediatric Surgery

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

The laboratory rat (Rattus norvegicus) is a key animal model for biomedical research. However, the genetic infrastructure required for connecting phenotype and genotype in the rat is currently incomplete. Here, we report the construction and integration of two genomic maps: a dense genetic linkage map of the rat and the first radiation hybrid (RH) map of the rat. The genetic map was constructed in two F2 intercrosses, containing a total of 4736 simple sequence length polymorphism (SSLP) markers. Allele sizes for 4328 of the genetic markers were characterized in 48 of the most commonly used inbred strains. The RH map is a lod >/= 3 framework map, including 983 SSLPs, thereby allowing integration with markers on various genetic maps and with markers mapped on the RH panel. Together, the maps provide an integrated reference to >3000 genes and ESTs and >8500 genetic markers.

Original language	English
Number of pages	8
Journal	Genome Research
Volume	9
Issue number	6
Early online date	21 May 1999
DOIs	https://doi.org/10.1101/gr.9.6.AP1
Publication status	Published - 1999

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Steen, R. G., Kwitek-Black, A. E., Glenn, C., Gullings-Handley, J., Van Etten, W., Atkinson, O. S., Appel, D., Twigger, S., Muir, M., Mull, T., Granados, M., Kissebah, M., Russo, K., Crane, R., Popp, M., Peden, M., Matise, T., Brown, D. M., Lu, J., ... Jacob, H. J. (1999). A high-density integrated genetic linkage and radiation hybrid map of the laboratory rat. Genome Research, 9(6). https://doi.org/10.1101/gr.9.6.AP1

@article{489d2fde7b99485cbe56c14ee408132e,

title = "A high-density integrated genetic linkage and radiation hybrid map of the laboratory rat",

abstract = "The laboratory rat (Rattus norvegicus) is a key animal model for biomedical research. However, the genetic infrastructure required for connecting phenotype and genotype in the rat is currently incomplete. Here, we report the construction and integration of two genomic maps: a dense genetic linkage map of the rat and the first radiation hybrid (RH) map of the rat. The genetic map was constructed in two F2 intercrosses, containing a total of 4736 simple sequence length polymorphism (SSLP) markers. Allele sizes for 4328 of the genetic markers were characterized in 48 of the most commonly used inbred strains. The RH map is a lod >/= 3 framework map, including 983 SSLPs, thereby allowing integration with markers on various genetic maps and with markers mapped on the RH panel. Together, the maps provide an integrated reference to >3000 genes and ESTs and >8500 genetic markers. ",

author = "Steen, {Robert G.} and Kwitek-Black, {Anne E.} and Christopher Glenn and Jo Gullings-Handley and {Van Etten}, William and Atkinson, {O. Scott} and Diane Appel and Simon Twigger and Melanie Muir and Tim Mull and Mary Granados and Mushira Kissebah and Kerri Russo and Robbin Crane and Michael Popp and Marc Peden and Tara Matise and Brown, {Donna M.} and Jian Lu and Stephen Kingsmore and Tonellato, {Peter J.} and Steve Rozen and Donna Slonim and Peter Young and Margit Knoblauch and Abraham Provoost and Detlev Ganten and Colman, {Steven D.} and Jonathan Rothberg and Lander, {Eric S.} and Jacob, {Howard J.}",

note = "{\textcopyright} 1999 by Cold Spring Harbor Laboratory Press",

year = "1999",

doi = "10.1101/gr.9.6.AP1",

language = "English",

volume = "9",

journal = "Genome Research",

issn = "1054-9803",

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Steen, RG, Kwitek-Black, AE, Glenn, C, Gullings-Handley, J, Van Etten, W, Atkinson, OS, Appel, D, Twigger, S, Muir, M, Mull, T, Granados, M, Kissebah, M, Russo, K, Crane, R, Popp, M, Peden, M, Matise, T, Brown, DM, Lu, J, Kingsmore, S, Tonellato, PJ, Rozen, S, Slonim, D, Young, P, Knoblauch, M, Provoost, A, Ganten, D, Colman, SD, Rothberg, J, Lander, ES & Jacob, HJ 1999, 'A high-density integrated genetic linkage and radiation hybrid map of the laboratory rat', Genome Research, vol. 9, no. 6. https://doi.org/10.1101/gr.9.6.AP1

TY - JOUR

T1 - A high-density integrated genetic linkage and radiation hybrid map of the laboratory rat

AU - Steen, Robert G.

AU - Kwitek-Black, Anne E.

AU - Glenn, Christopher

AU - Gullings-Handley, Jo

AU - Van Etten, William

AU - Atkinson, O. Scott

AU - Appel, Diane

AU - Twigger, Simon

AU - Muir, Melanie

AU - Mull, Tim

AU - Granados, Mary

AU - Kissebah, Mushira

AU - Russo, Kerri

AU - Crane, Robbin

AU - Popp, Michael

AU - Peden, Marc

AU - Matise, Tara

AU - Brown, Donna M.

AU - Lu, Jian

AU - Kingsmore, Stephen

AU - Tonellato, Peter J.

AU - Rozen, Steve

AU - Slonim, Donna

AU - Young, Peter

AU - Knoblauch, Margit

AU - Provoost, Abraham

AU - Ganten, Detlev

AU - Colman, Steven D.

AU - Rothberg, Jonathan

AU - Lander, Eric S.

AU - Jacob, Howard J.

PY - 1999

Y1 - 1999

N2 - The laboratory rat (Rattus norvegicus) is a key animal model for biomedical research. However, the genetic infrastructure required for connecting phenotype and genotype in the rat is currently incomplete. Here, we report the construction and integration of two genomic maps: a dense genetic linkage map of the rat and the first radiation hybrid (RH) map of the rat. The genetic map was constructed in two F2 intercrosses, containing a total of 4736 simple sequence length polymorphism (SSLP) markers. Allele sizes for 4328 of the genetic markers were characterized in 48 of the most commonly used inbred strains. The RH map is a lod >/= 3 framework map, including 983 SSLPs, thereby allowing integration with markers on various genetic maps and with markers mapped on the RH panel. Together, the maps provide an integrated reference to >3000 genes and ESTs and >8500 genetic markers.

AB - The laboratory rat (Rattus norvegicus) is a key animal model for biomedical research. However, the genetic infrastructure required for connecting phenotype and genotype in the rat is currently incomplete. Here, we report the construction and integration of two genomic maps: a dense genetic linkage map of the rat and the first radiation hybrid (RH) map of the rat. The genetic map was constructed in two F2 intercrosses, containing a total of 4736 simple sequence length polymorphism (SSLP) markers. Allele sizes for 4328 of the genetic markers were characterized in 48 of the most commonly used inbred strains. The RH map is a lod >/= 3 framework map, including 983 SSLPs, thereby allowing integration with markers on various genetic maps and with markers mapped on the RH panel. Together, the maps provide an integrated reference to >3000 genes and ESTs and >8500 genetic markers.

UR - https://www.researchgate.net/publication/12898243_A_High-Density_Integrated_Genetic_Linkage_and_Radiation_Hybrid_Map_of_the_Laboratory_Rat

U2 - 10.1101/gr.9.6.AP1

DO - 10.1101/gr.9.6.AP1

M3 - Article

C2 - 10400928

SN - 1054-9803

VL - 9

JO - Genome Research

JF - Genome Research

IS - 6

ER -

A high-density integrated genetic linkage and radiation hybrid map of the laboratory rat

Abstract

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