Abstract
Neural stem cell implantations have been extensively investigated for treatment of brain diseases such as stroke. In order to follow the localization and functional status of cells after implantation noninvasive imaging is essential. Therefore, we developed a comprehensive multi-modality platform for invivo imaging of graft localization, density, and survival using 19F magnetic resonance imaging in combination with bioluminescence imaging. We quantitatively analyzed cell graft survival over the first 4 weeks after transplantation in both healthy and stroke-damaged mouse brain and correlated our findings of graft vitality with the host innate immune response. The multi-modality imaging platform will help to improve cell therapy also in context other than stroke and to gain indispensable information for clinical translation.
| Original language | English |
|---|---|
| Pages (from-to) | 2218-2226 |
| Number of pages | 9 |
| Journal | Biomaterials |
| Volume | 35 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published - Feb 2014 |
| Externally published | Yes |
Bibliographical note
Funding Information:The authors thank Nadine Henn and Gabriele Schneider for excellent technical support, Laura Breucker and Annette Tennstädt for help with a pilot study and Stefan Wecker and Wilfried Haider for support with the MRI hardware setup. Generous supply of CS1000 green by C. O'Hanlon from Celsense Inc. is gratefully acknowledged. This work was financially supported by grants from the Volkswagen Foundation (I/83 443), the EU-FP7 program ENCITE (HEALTH-F5-2008-201842) and TargetBraIn (HEALTH-F2-2012-279017). The funding sources had no role in study design, in the collection, analysis and interpretation of data, in the writing of the report, and in the decision to submit the paper for publication.