A New Technology for Stabilization of Biomolecules in Tissues for Combined Histological and Molecular Analyses

C Viertler, D Groelz, S Gundisch, K Kashofer, B Reischauer, Peter Riegman, R Winther, R Wyrich, KF Becker, U Oelmuller, K Zatloukal

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70 Citations (Scopus)

Abstract

For accurate diagnosis, prediction of outcome, and selection of appropriate therapies, the molecular characterization of human diseases requires analysis of a broad spectrum of altered biomolecules, in addition to morphological features, in affected tissues such as tumors. In a high-throughput screening approach, we have developed the PAXgene Tissue System as a novel tissue stabilization technology. Comprehensive characterization of this technology in stabilized and paraffin-embedded human tissues and comparison with snap-frozen tissues revealed excellent preservation of morphology and antigenicity, as well as outstanding Integrity of nucleic acids (genomic DNA, miRNA, and mRNA) and phosphoproteins. Importantly, PAXgene-fixed, paraffin-embedded tissues provided RNA quantity and quality not only significantly better than that obtained with neutral buffered formalin, but also similar to that from snap-frozen tissue, which currently represents the gold standard for molecular analyses. The PAXgene tissue stabilization system thus opens new opportunities in a variety of molecular diagnostic and research applications in which the collection of snap-frozen tissue is not feasible for medical, logistic, or ethical reasons. Furthermore, this technology allows performing histopathological analyses together with molecular studies in a single sample, which markedly facilitates direct correlation of morphological disease phenotypes with alterations of nucleic acids and other biomolecules. (J Mol Diagn 2012, 14:458-466. http://dx.doi.org/10.1016/j.jmoldx.2012.05.002)
Original languageUndefined/Unknown
Pages (from-to)458-466
Number of pages9
JournalJournal of Molecular Diagnostics
Volume14
Issue number5
DOIs
Publication statusPublished - 2012

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