A new ultrafast and high-throughput mass spectrometric approach for the therapeutic drug monitoring of the multi-targeted anti-folate pemetrexed in plasma from lung cancer patients

Roland Meesters, Robin Cornelissen, RJ Klaveren, R Jonge, E Boer, Jan Lindemans, Theo Luider

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An analytical assay has been developed and validated for ultrafast and high-throughput mass spectrometric determination of pemetrexed concentrations in plasma using matrix assisted laser desorption/ionization-triple quadrupole-tandem mass spectrometry. Patient plasma samples spiked with the internal standard methotrexate were measured by multiple reaction monitoring. The detection limit was 0.4 fmol/mu L, lower limit of quantification was 0.9 fmol/mu L, and upper limit of quantification was 60 fmol/mu L, respectively. Overall observed pemetrexed concentrations in patient samples ranged between 8.7 (1.4) and 142.7 (20.3) pmol/mu L (SD). The newly developed mass spectrometric assay is applicable for (routine) therapeutic drug monitoring of pemetrexed concentrations in plasma from non-small cell lung cancer patients.
Original languageUndefined/Unknown
Pages (from-to)2943-2948
Number of pages6
JournalAnalytical and Bioanalytical Chemistry
Issue number7-8
Publication statusPublished - 2010

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