A novel clinical grade isolation method for human kidney perivascular stromal cells

Daniëlle G. Leuning; Ellen Lievers; Marlies E.J. Reinders; Cees van Kooten; Marten A. Engelse; Ton J. Rabelink

doi:10.3791/55841

A novel clinical grade isolation method for human kidney perivascular stromal cells

Daniëlle G. Leuning, Ellen Lievers, Marlies E.J. Reinders, Cees van Kooten, Marten A. Engelse, Ton J. Rabelink^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

2 Citations (Scopus)

1 Downloads (Pure)

Abstract

Mesenchymal Stromal Cells (MSCs) are tissue homeostatic and immune modulatory cells that have shown beneficial effects in kidney diseases and transplantation. Perivascular Stromal Cells (PSCs) share characteristics with bone marrow MSCs (bmMSCs). However, they also possess, most likely due to local imprinting, tissue-specific properties and play a role in local tissue homeostasis. This tissue specificity may result in tissue specific repair, also within the human kidney. We previously showed that human kidney PSCs (kPSCs) have enhanced kidney epithelial wound healing whereas bmMSCs did not have this potential. Moreover, kPSCs can ameliorate kidney injury in vivo. Therefore, kPSCs constitute an interesting source for cell therapy, particularly for kidney diseases and renal transplantation. Here we show the detailed isolation and culture method for kPSCs from transplant-grade human kidneys based on whole-organ perfusion of digestive enzymes via the renal artery and enrichment for the perivascular marker NG2. In this way, large cell quantities can be obtained that are suitable for cellular therapy.

Original language	English
Article number	e55841
Journal	Journal of Visualized Experiments
Volume	2017
Issue number	126
DOIs	https://doi.org/10.3791/55841
Publication status	Published - 7 Aug 2017
Externally published	Yes

Bibliographical note

Funding Information:
This research has received funding from the European Community’s Seventh Framework Program (FP7/2007-2013) under grant agreement number 305436 STELLAR.

Publisher Copyright:
© 2017 Journal of Visualized Experiments.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.3791/55841Licence: CC BY-NC-ND

A Novel Clinical Grade Isolation Method for Human Kidney Perivascular Stromal CellsFinal published version, 558 KBLicence: CC BY-NC-ND

Cite this

@article{fd0f3d834cb541e786b5e162177bd54d,

title = "A novel clinical grade isolation method for human kidney perivascular stromal cells",

abstract = "Mesenchymal Stromal Cells (MSCs) are tissue homeostatic and immune modulatory cells that have shown beneficial effects in kidney diseases and transplantation. Perivascular Stromal Cells (PSCs) share characteristics with bone marrow MSCs (bmMSCs). However, they also possess, most likely due to local imprinting, tissue-specific properties and play a role in local tissue homeostasis. This tissue specificity may result in tissue specific repair, also within the human kidney. We previously showed that human kidney PSCs (kPSCs) have enhanced kidney epithelial wound healing whereas bmMSCs did not have this potential. Moreover, kPSCs can ameliorate kidney injury in vivo. Therefore, kPSCs constitute an interesting source for cell therapy, particularly for kidney diseases and renal transplantation. Here we show the detailed isolation and culture method for kPSCs from transplant-grade human kidneys based on whole-organ perfusion of digestive enzymes via the renal artery and enrichment for the perivascular marker NG2. In this way, large cell quantities can be obtained that are suitable for cellular therapy.",

author = "Leuning, {Dani{\"e}lle G.} and Ellen Lievers and Reinders, {Marlies E.J.} and {van Kooten}, Cees and Engelse, {Marten A.} and Rabelink, {Ton J.}",

note = "Funding Information: This research has received funding from the European Community{\textquoteright}s Seventh Framework Program (FP7/2007-2013) under grant agreement number 305436 STELLAR. Publisher Copyright: {\textcopyright} 2017 Journal of Visualized Experiments.",

year = "2017",

month = aug,

day = "7",

doi = "10.3791/55841",

language = "English",

volume = "2017",

journal = "Journal of Visualized Experiments",

issn = "1940-087X",

publisher = "MYJoVE Corporation",

number = "126",

}

TY - JOUR

T1 - A novel clinical grade isolation method for human kidney perivascular stromal cells

AU - Leuning, Daniëlle G.

AU - Lievers, Ellen

AU - Reinders, Marlies E.J.

AU - van Kooten, Cees

AU - Engelse, Marten A.

AU - Rabelink, Ton J.

N1 - Funding Information: This research has received funding from the European Community’s Seventh Framework Program (FP7/2007-2013) under grant agreement number 305436 STELLAR. Publisher Copyright: © 2017 Journal of Visualized Experiments.

PY - 2017/8/7

Y1 - 2017/8/7

N2 - Mesenchymal Stromal Cells (MSCs) are tissue homeostatic and immune modulatory cells that have shown beneficial effects in kidney diseases and transplantation. Perivascular Stromal Cells (PSCs) share characteristics with bone marrow MSCs (bmMSCs). However, they also possess, most likely due to local imprinting, tissue-specific properties and play a role in local tissue homeostasis. This tissue specificity may result in tissue specific repair, also within the human kidney. We previously showed that human kidney PSCs (kPSCs) have enhanced kidney epithelial wound healing whereas bmMSCs did not have this potential. Moreover, kPSCs can ameliorate kidney injury in vivo. Therefore, kPSCs constitute an interesting source for cell therapy, particularly for kidney diseases and renal transplantation. Here we show the detailed isolation and culture method for kPSCs from transplant-grade human kidneys based on whole-organ perfusion of digestive enzymes via the renal artery and enrichment for the perivascular marker NG2. In this way, large cell quantities can be obtained that are suitable for cellular therapy.

AB - Mesenchymal Stromal Cells (MSCs) are tissue homeostatic and immune modulatory cells that have shown beneficial effects in kidney diseases and transplantation. Perivascular Stromal Cells (PSCs) share characteristics with bone marrow MSCs (bmMSCs). However, they also possess, most likely due to local imprinting, tissue-specific properties and play a role in local tissue homeostasis. This tissue specificity may result in tissue specific repair, also within the human kidney. We previously showed that human kidney PSCs (kPSCs) have enhanced kidney epithelial wound healing whereas bmMSCs did not have this potential. Moreover, kPSCs can ameliorate kidney injury in vivo. Therefore, kPSCs constitute an interesting source for cell therapy, particularly for kidney diseases and renal transplantation. Here we show the detailed isolation and culture method for kPSCs from transplant-grade human kidneys based on whole-organ perfusion of digestive enzymes via the renal artery and enrichment for the perivascular marker NG2. In this way, large cell quantities can be obtained that are suitable for cellular therapy.

UR - http://www.scopus.com/inward/record.url?scp=85027025638&partnerID=8YFLogxK

U2 - 10.3791/55841

DO - 10.3791/55841

M3 - Article

C2 - 28809840

AN - SCOPUS:85027025638

SN - 1940-087X

VL - 2017

JO - Journal of Visualized Experiments

JF - Journal of Visualized Experiments

IS - 126

M1 - e55841

ER -

A novel clinical grade isolation method for human kidney perivascular stromal cells

Abstract

Bibliographical note

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this