A novel clinical grade isolation method for human kidney perivascular stromal cells

Daniëlle G. Leuning, Ellen Lievers, Marlies E.J. Reinders, Cees van Kooten, Marten A. Engelse, Ton J. Rabelink*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

2 Citations (Scopus)
1 Downloads (Pure)


Mesenchymal Stromal Cells (MSCs) are tissue homeostatic and immune modulatory cells that have shown beneficial effects in kidney diseases and transplantation. Perivascular Stromal Cells (PSCs) share characteristics with bone marrow MSCs (bmMSCs). However, they also possess, most likely due to local imprinting, tissue-specific properties and play a role in local tissue homeostasis. This tissue specificity may result in tissue specific repair, also within the human kidney. We previously showed that human kidney PSCs (kPSCs) have enhanced kidney epithelial wound healing whereas bmMSCs did not have this potential. Moreover, kPSCs can ameliorate kidney injury in vivo. Therefore, kPSCs constitute an interesting source for cell therapy, particularly for kidney diseases and renal transplantation. Here we show the detailed isolation and culture method for kPSCs from transplant-grade human kidneys based on whole-organ perfusion of digestive enzymes via the renal artery and enrichment for the perivascular marker NG2. In this way, large cell quantities can be obtained that are suitable for cellular therapy.

Original languageEnglish
Article numbere55841
JournalJournal of Visualized Experiments
Issue number126
Publication statusPublished - 7 Aug 2017
Externally publishedYes

Bibliographical note

Funding Information:
This research has received funding from the European Community’s Seventh Framework Program (FP7/2007-2013) under grant agreement number 305436 STELLAR.

Publisher Copyright:
© 2017 Journal of Visualized Experiments.


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