A Novel Y319H Substitution in CYP51C Associated with Azole Resistance in Aspergillus flavus

RA Paul; SM Rudramurthy; Jacobus Meis; Johan Mouton; A Chakrabarti

doi:10.1128/AAC.00637-15

A Novel Y319H Substitution in CYP51C Associated with Azole Resistance in Aspergillus flavus

RA Paul, SM Rudramurthy, Jacobus Meis, Johan Mouton, A Chakrabarti

Medical Microbiology & Infectious Diseases

Research output: Contribution to journal › Article › Academic › peer-review

61 Citations (Scopus)

Abstract

This study aimed to explore any mutation in the CYP51 gene conferring azole resistance in Aspergillus flavus. Two voriconazole-resistant and 45 voriconazole-susceptible isolates were included in the study. Sequence analysis demonstrated a T1025C nucleotide change in CYP51C, resulting in the Y319H amino acid substitution in one resistant isolate. However, the earlier described T788G mutation in CYP51C conferring voriconazole resistance in A. flavus isolates was present in all isolates, irrespective of their susceptibility status.

Original language	Undefined/Unknown
Pages (from-to)	6615-6619
Number of pages	5
Journal	Antimicrobial Agents & Chemotherapy
Volume	59
Issue number	10
DOIs	https://doi.org/10.1128/AAC.00637-15
Publication status	Published - 2015

Research programs

EMC MM-04-28-01

Access to Document

10.1128/AAC.00637-15

http://hdl.handle.net/1765/81672

Cite this

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abstract = "This study aimed to explore any mutation in the CYP51 gene conferring azole resistance in Aspergillus flavus. Two voriconazole-resistant and 45 voriconazole-susceptible isolates were included in the study. Sequence analysis demonstrated a T1025C nucleotide change in CYP51C, resulting in the Y319H amino acid substitution in one resistant isolate. However, the earlier described T788G mutation in CYP51C conferring voriconazole resistance in A. flavus isolates was present in all isolates, irrespective of their susceptibility status.",

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AB - This study aimed to explore any mutation in the CYP51 gene conferring azole resistance in Aspergillus flavus. Two voriconazole-resistant and 45 voriconazole-susceptible isolates were included in the study. Sequence analysis demonstrated a T1025C nucleotide change in CYP51C, resulting in the Y319H amino acid substitution in one resistant isolate. However, the earlier described T788G mutation in CYP51C conferring voriconazole resistance in A. flavus isolates was present in all isolates, irrespective of their susceptibility status.

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