A pictorial essay on fetal rabbit anatomy using micro-ultrasound and magnetic resonance imaging

Philip DeKoninck; Masayuki Endo; Inga Sandaite; Jute Richter; Luc De Catte; Ben Van Calster; Jaan Toelen; Uwe Himmelreich; Filip Claus; Jan Deprest

doi:10.1002/pd.4259

A pictorial essay on fetal rabbit anatomy using micro-ultrasound and magnetic resonance imaging

Philip DeKoninck, Masayuki Endo, Inga Sandaite, Jute Richter, Luc De Catte, Ben Van Calster, Jaan Toelen, Uwe Himmelreich, Filip Claus, Jan Deprest^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

5 Citations (Scopus)

Abstract

INTRODUCTION: With this pictorial essay, we aimed to provide gestational age specific reference ranges of relevant fetal structures using micro-ultrasound, as well as its correlation with postmortem MRI and whole body sections.

MATERIAL AND METHODS: Time-mated pregnant rabbits (n = 24) were assessed once at various gestational ages in the second half of pregnancy (15, 17, 21 to 23, 25 to 28, and 30 to 31 days; term = 31 days). We obtained biometric data, together with Doppler flow patterns in the ductus venosus, umbilical artery and umbilical vein. After euthanasia, micro-ultrasound images were compared with images obtained by 9.4 Tesla MRI and whole body paraffin sections at 15, 23, 26, and 28 days.

RESULTS: We constructed biometric normative curves, which showed a significant correlation with gestational age. The pulsatility index (PI) in the umbilical artery decreased with gestation (PI = 5.746-0.2969(GA) + 0.004931(GA)(2) ; R(2) = 0.30), whereas pulsatility index for veins (PIV) in the ductus venosus remained constant (median PIV = 0.82 (0.60-1.12)). In this report, we provide an anatomical atlas of fetal thoracic development using both micro-ultrasound and MRI.

CONCLUSION: We describe anatomical fetal leporine development as can be visualized by micro-ultrasound and MR imaging. The reported reference ranges may be useful for researchers using the fetal rabbit model.

Original language	English
Pages (from-to)	84-89
Number of pages	6
Journal	Prenatal Diagnosis
Volume	34
Issue number	1
DOIs	https://doi.org/10.1002/pd.4259
Publication status	Published - Jan 2014
Externally published	Yes

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Cite this

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title = "A pictorial essay on fetal rabbit anatomy using micro-ultrasound and magnetic resonance imaging",

abstract = "INTRODUCTION: With this pictorial essay, we aimed to provide gestational age specific reference ranges of relevant fetal structures using micro-ultrasound, as well as its correlation with postmortem MRI and whole body sections.MATERIAL AND METHODS: Time-mated pregnant rabbits (n = 24) were assessed once at various gestational ages in the second half of pregnancy (15, 17, 21 to 23, 25 to 28, and 30 to 31 days; term = 31 days). We obtained biometric data, together with Doppler flow patterns in the ductus venosus, umbilical artery and umbilical vein. After euthanasia, micro-ultrasound images were compared with images obtained by 9.4 Tesla MRI and whole body paraffin sections at 15, 23, 26, and 28 days.RESULTS: We constructed biometric normative curves, which showed a significant correlation with gestational age. The pulsatility index (PI) in the umbilical artery decreased with gestation (PI = 5.746-0.2969(GA) + 0.004931(GA)(2) ; R(2) = 0.30), whereas pulsatility index for veins (PIV) in the ductus venosus remained constant (median PIV = 0.82 (0.60-1.12)). In this report, we provide an anatomical atlas of fetal thoracic development using both micro-ultrasound and MRI.CONCLUSION: We describe anatomical fetal leporine development as can be visualized by micro-ultrasound and MR imaging. The reported reference ranges may be useful for researchers using the fetal rabbit model.",

author = "Philip DeKoninck and Masayuki Endo and Inga Sandaite and Jute Richter and {De Catte}, Luc and {Van Calster}, Ben and Jaan Toelen and Uwe Himmelreich and Filip Claus and Jan Deprest",

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year = "2014",

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doi = "10.1002/pd.4259",

language = "English",

volume = "34",

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AU - DeKoninck, Philip

AU - Endo, Masayuki

AU - Sandaite, Inga

AU - Richter, Jute

AU - De Catte, Luc

AU - Van Calster, Ben

AU - Toelen, Jaan

AU - Himmelreich, Uwe

AU - Claus, Filip

AU - Deprest, Jan

PY - 2014/1

Y1 - 2014/1

N2 - INTRODUCTION: With this pictorial essay, we aimed to provide gestational age specific reference ranges of relevant fetal structures using micro-ultrasound, as well as its correlation with postmortem MRI and whole body sections.MATERIAL AND METHODS: Time-mated pregnant rabbits (n = 24) were assessed once at various gestational ages in the second half of pregnancy (15, 17, 21 to 23, 25 to 28, and 30 to 31 days; term = 31 days). We obtained biometric data, together with Doppler flow patterns in the ductus venosus, umbilical artery and umbilical vein. After euthanasia, micro-ultrasound images were compared with images obtained by 9.4 Tesla MRI and whole body paraffin sections at 15, 23, 26, and 28 days.RESULTS: We constructed biometric normative curves, which showed a significant correlation with gestational age. The pulsatility index (PI) in the umbilical artery decreased with gestation (PI = 5.746-0.2969(GA) + 0.004931(GA)(2) ; R(2) = 0.30), whereas pulsatility index for veins (PIV) in the ductus venosus remained constant (median PIV = 0.82 (0.60-1.12)). In this report, we provide an anatomical atlas of fetal thoracic development using both micro-ultrasound and MRI.CONCLUSION: We describe anatomical fetal leporine development as can be visualized by micro-ultrasound and MR imaging. The reported reference ranges may be useful for researchers using the fetal rabbit model.

AB - INTRODUCTION: With this pictorial essay, we aimed to provide gestational age specific reference ranges of relevant fetal structures using micro-ultrasound, as well as its correlation with postmortem MRI and whole body sections.MATERIAL AND METHODS: Time-mated pregnant rabbits (n = 24) were assessed once at various gestational ages in the second half of pregnancy (15, 17, 21 to 23, 25 to 28, and 30 to 31 days; term = 31 days). We obtained biometric data, together with Doppler flow patterns in the ductus venosus, umbilical artery and umbilical vein. After euthanasia, micro-ultrasound images were compared with images obtained by 9.4 Tesla MRI and whole body paraffin sections at 15, 23, 26, and 28 days.RESULTS: We constructed biometric normative curves, which showed a significant correlation with gestational age. The pulsatility index (PI) in the umbilical artery decreased with gestation (PI = 5.746-0.2969(GA) + 0.004931(GA)(2) ; R(2) = 0.30), whereas pulsatility index for veins (PIV) in the ductus venosus remained constant (median PIV = 0.82 (0.60-1.12)). In this report, we provide an anatomical atlas of fetal thoracic development using both micro-ultrasound and MRI.CONCLUSION: We describe anatomical fetal leporine development as can be visualized by micro-ultrasound and MR imaging. The reported reference ranges may be useful for researchers using the fetal rabbit model.

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DO - 10.1002/pd.4259

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JO - Prenatal Diagnosis

JF - Prenatal Diagnosis

IS - 1

ER -

A pictorial essay on fetal rabbit anatomy using micro-ultrasound and magnetic resonance imaging

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