A plasma creatinine- and urea-based equation to estimate glomerular filtration rate in rats

Paul J. Besseling, Tobias T. Pieters*, Isabel T. N. Nguyen, Petra M. de Bree, Nel Willekes, Adele H. Dijk, Dominique M. Bovee, Ewout J. Hoorn, Maarten B. Rookmaaker, Karin G. Gerritsen, Marianne C. Verhaar, Hendrik Gremmels, Jaap A. Joles

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

21 Citations (Scopus)


Monitoring renal function is a vital part of kidney research involving rats. The laborious measurement of glomerular filtration rate (GFR) with administration of exogenous filtration markers does not easily allow serial measurements. Using an in-house database of inulin clearances, we developed and validated a plasma creatinine- and plasma urea-based equation to estimate GFR in a large cohort of male rats [development cohort n = 325, R2 = 0.816, percentage of predictions that fell within 30% of the true value (P30) = 76%] that had high accuracy in the validation cohort (n = 116 rats, R2 = 0.935, P30 = 79%). The equation was less accurate in rats with nonsteady-state creatinine, in which the equation should therefore not be used. In conclusion, applying this equation facilitates easy and repeatable estimates of GFR in rats. NEW & NOTEWORTHY This is the first equation, that we know of, which estimates glomerular filtration rate in rats based on a single measurement of body weight, plasma creatinine, and plasma urea.

Original languageEnglish
Pages (from-to)F518-F524
JournalAmerican Journal of Physiology-Renal Physiology
Issue number3
Publication statusPublished - 12 Mar 2021

Bibliographical note

Funding Information:
This work was supported by The Netherlands CardioVascular Research Initiative: an initiative with support of the Dutch Heart Foundation [Grant No. CVON2014-11 (RECONNECT)], by ZonMw within the LSH 2Treat programme and the Dutch Kidney Foundation [Grant No. 436001003 (InSiTeVx)], and by the Alexandre Suerman Stipend from UMC Utrecht.

Publisher Copyright:
© 2021 the American Physiological Society


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