A plasma creatinine- and urea-based equation to estimate glomerular filtration rate in rats

Paul J. Besseling; Tobias T. Pieters; Isabel T. N. Nguyen; Petra M. de Bree; Nel Willekes; Adele H. Dijk; Dominique M. Bovee; Ewout J. Hoorn; Maarten B. Rookmaaker; Karin G. Gerritsen; Marianne C. Verhaar; Hendrik Gremmels; Jaap A. Joles

doi:10.1152/ajprenal.00656.2020

A plasma creatinine- and urea-based equation to estimate glomerular filtration rate in rats

Paul J. Besseling, Tobias T. Pieters^*, Isabel T. N. Nguyen, Petra M. de Bree, Nel Willekes, Adele H. Dijk, Dominique M. Bovee, Ewout J. Hoorn, Maarten B. Rookmaaker, Karin G. Gerritsen, Marianne C. Verhaar, Hendrik Gremmels, Jaap A. Joles

^*Corresponding author for this work

Internal Medicine

Research output: Contribution to journal › Article › Academic › peer-review

21 Citations (Scopus)

Abstract

Monitoring renal function is a vital part of kidney research involving rats. The laborious measurement of glomerular filtration rate (GFR) with administration of exogenous filtration markers does not easily allow serial measurements. Using an in-house database of inulin clearances, we developed and validated a plasma creatinine- and plasma urea-based equation to estimate GFR in a large cohort of male rats [development cohort n = 325, R² = 0.816, percentage of predictions that fell within 30% of the true value (P30) = 76%] that had high accuracy in the validation cohort (n = 116 rats, R² = 0.935, P30 = 79%). The equation was less accurate in rats with nonsteady-state creatinine, in which the equation should therefore not be used. In conclusion, applying this equation facilitates easy and repeatable estimates of GFR in rats. NEW & NOTEWORTHY This is the first equation, that we know of, which estimates glomerular filtration rate in rats based on a single measurement of body weight, plasma creatinine, and plasma urea.

Original language	English
Pages (from-to)	F518-F524
Journal	American Journal of Physiology-Renal Physiology
Volume	320
Issue number	3
DOIs	https://doi.org/10.1152/ajprenal.00656.2020 https://doi.org/10.1152/ajprenal.00656.2020
Publication status	Published - 12 Mar 2021

Bibliographical note

Funding Information:
This work was supported by The Netherlands CardioVascular Research Initiative: an initiative with support of the Dutch Heart Foundation [Grant No. CVON2014-11 (RECONNECT)], by ZonMw within the LSH 2Treat programme and the Dutch Kidney Foundation [Grant No. 436001003 (InSiTeVx)], and by the Alexandre Suerman Stipend from UMC Utrecht.

Publisher Copyright:
© 2021 the American Physiological Society

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Besseling, P. J., Pieters, T. T., Nguyen, I. T. N., de Bree, P. M., Willekes, N., Dijk, A. H., Bovee, D. M., Hoorn, E. J., Rookmaaker, M. B., Gerritsen, K. G., Verhaar, M. C., Gremmels, H., & Joles, J. A. (2021). A plasma creatinine- and urea-based equation to estimate glomerular filtration rate in rats. American Journal of Physiology-Renal Physiology, 320(3), F518-F524. https://doi.org/10.1152/ajprenal.00656.2020, https://doi.org/10.1152/ajprenal.00656.2020

@article{bc4d76571c0b43fca16beac5d07d098d,

title = "A plasma creatinine- and urea-based equation to estimate glomerular filtration rate in rats",

abstract = "Monitoring renal function is a vital part of kidney research involving rats. The laborious measurement of glomerular filtration rate (GFR) with administration of exogenous filtration markers does not easily allow serial measurements. Using an in-house database of inulin clearances, we developed and validated a plasma creatinine- and plasma urea-based equation to estimate GFR in a large cohort of male rats [development cohort n = 325, R2 = 0.816, percentage of predictions that fell within 30% of the true value (P30) = 76%] that had high accuracy in the validation cohort (n = 116 rats, R2 = 0.935, P30 = 79%). The equation was less accurate in rats with nonsteady-state creatinine, in which the equation should therefore not be used. In conclusion, applying this equation facilitates easy and repeatable estimates of GFR in rats. NEW & NOTEWORTHY This is the first equation, that we know of, which estimates glomerular filtration rate in rats based on a single measurement of body weight, plasma creatinine, and plasma urea.",

author = "Besseling, {Paul J.} and Pieters, {Tobias T.} and Nguyen, {Isabel T. N.} and {de Bree}, {Petra M.} and Nel Willekes and Dijk, {Adele H.} and Bovee, {Dominique M.} and Hoorn, {Ewout J.} and Rookmaaker, {Maarten B.} and Gerritsen, {Karin G.} and Verhaar, {Marianne C.} and Hendrik Gremmels and Joles, {Jaap A.}",

note = "Funding Information: This work was supported by The Netherlands CardioVascular Research Initiative: an initiative with support of the Dutch Heart Foundation [Grant No. CVON2014-11 (RECONNECT)], by ZonMw within the LSH 2Treat programme and the Dutch Kidney Foundation [Grant No. 436001003 (InSiTeVx)], and by the Alexandre Suerman Stipend from UMC Utrecht. Publisher Copyright: {\textcopyright} 2021 the American Physiological Society",

year = "2021",

month = mar,

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doi = "10.1152/ajprenal.00656.2020",

language = "English",

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Besseling, PJ, Pieters, TT, Nguyen, ITN, de Bree, PM, Willekes, N, Dijk, AH, Bovee, DM , Hoorn, EJ, Rookmaaker, MB, Gerritsen, KG, Verhaar, MC, Gremmels, H & Joles, JA 2021, 'A plasma creatinine- and urea-based equation to estimate glomerular filtration rate in rats', American Journal of Physiology-Renal Physiology, vol. 320, no. 3, pp. F518-F524. https://doi.org/10.1152/ajprenal.00656.2020, https://doi.org/10.1152/ajprenal.00656.2020

TY - JOUR

T1 - A plasma creatinine- and urea-based equation to estimate glomerular filtration rate in rats

AU - Besseling, Paul J.

AU - Pieters, Tobias T.

AU - Nguyen, Isabel T. N.

AU - de Bree, Petra M.

AU - Willekes, Nel

AU - Dijk, Adele H.

AU - Bovee, Dominique M.

AU - Hoorn, Ewout J.

AU - Rookmaaker, Maarten B.

AU - Gerritsen, Karin G.

AU - Verhaar, Marianne C.

AU - Gremmels, Hendrik

AU - Joles, Jaap A.

N1 - Funding Information: This work was supported by The Netherlands CardioVascular Research Initiative: an initiative with support of the Dutch Heart Foundation [Grant No. CVON2014-11 (RECONNECT)], by ZonMw within the LSH 2Treat programme and the Dutch Kidney Foundation [Grant No. 436001003 (InSiTeVx)], and by the Alexandre Suerman Stipend from UMC Utrecht. Publisher Copyright: © 2021 the American Physiological Society

PY - 2021/3/12

Y1 - 2021/3/12

N2 - Monitoring renal function is a vital part of kidney research involving rats. The laborious measurement of glomerular filtration rate (GFR) with administration of exogenous filtration markers does not easily allow serial measurements. Using an in-house database of inulin clearances, we developed and validated a plasma creatinine- and plasma urea-based equation to estimate GFR in a large cohort of male rats [development cohort n = 325, R2 = 0.816, percentage of predictions that fell within 30% of the true value (P30) = 76%] that had high accuracy in the validation cohort (n = 116 rats, R2 = 0.935, P30 = 79%). The equation was less accurate in rats with nonsteady-state creatinine, in which the equation should therefore not be used. In conclusion, applying this equation facilitates easy and repeatable estimates of GFR in rats. NEW & NOTEWORTHY This is the first equation, that we know of, which estimates glomerular filtration rate in rats based on a single measurement of body weight, plasma creatinine, and plasma urea.

AB - Monitoring renal function is a vital part of kidney research involving rats. The laborious measurement of glomerular filtration rate (GFR) with administration of exogenous filtration markers does not easily allow serial measurements. Using an in-house database of inulin clearances, we developed and validated a plasma creatinine- and plasma urea-based equation to estimate GFR in a large cohort of male rats [development cohort n = 325, R2 = 0.816, percentage of predictions that fell within 30% of the true value (P30) = 76%] that had high accuracy in the validation cohort (n = 116 rats, R2 = 0.935, P30 = 79%). The equation was less accurate in rats with nonsteady-state creatinine, in which the equation should therefore not be used. In conclusion, applying this equation facilitates easy and repeatable estimates of GFR in rats. NEW & NOTEWORTHY This is the first equation, that we know of, which estimates glomerular filtration rate in rats based on a single measurement of body weight, plasma creatinine, and plasma urea.

UR - http://www.scopus.com/inward/record.url?scp=85102964212&partnerID=8YFLogxK

U2 - 10.1152/ajprenal.00656.2020

DO - 10.1152/ajprenal.00656.2020

M3 - Article

SN - 1931-857X

VL - 320

SP - F518-F524

JO - American Journal of Physiology-Renal Physiology

JF - American Journal of Physiology-Renal Physiology

IS - 3

ER -

A plasma creatinine- and urea-based equation to estimate glomerular filtration rate in rats

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