A simplified protocol for the generation of cortical brain organoids

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Human brain organoid technology has the potential to generate unprecedented insight into normal and aberrant brain development. It opens up a developmental time window in which the effects of gene or environmental perturbations can be experimentally tested. However, detection sensitivity and correct interpretation of phenotypes are hampered by notable batch-to-batch variability and low reproducibility of cell and regional identities. Here, we describe a detailed, simplified protocol for the robust and reproducible generation of brain organoids with cortical identity from feeder-independent induced pluripotent stem cells (iPSCs). This self-patterning approach minimizes media supplements and handling steps, resulting in cortical brain organoids that can be maintained over prolonged periods and that contain radial glial and intermediate progenitors, deep and upper layer neurons, and astrocytes.

Original languageEnglish
Article number1114420
JournalFrontiers in Cellular Neuroscience
Publication statusPublished - 4 Apr 2023

Bibliographical note

Funding Information:
DB is supported by a Horizon 2020 Marie Curie Individual Fellowship (#799214) and an Erasmus University Rotterdam Fellowship. FV is supported by the Erasmus MC Human Disease Model Award. FV and SK are supported by the Netherlands Organ-on-Chip Initiative, an NWO Gravitation project (024.003.001) funded by the Ministry of Education, Culture, and Science of the Government of the Netherlands.

Publisher Copyright:
Copyright © 2023 Eigenhuis, Somsen, van der Kroeg, Smeenk, Korporaal, Kushner, de Vrij and van den Berg.


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