A snapshot of Plasmodium falciparum malaria drug resistance markers in Sudan: a pilot study

Nouh S. Mohamed; Hanadi Abdelbagi; Hussam A. Osman; Abdallah E. Ahmed; Alaa M. Yousif; Yusraa B. Edris; Eman Y. Osman; Aahd R. Elsadig; Emmanuel E. Siddig; Madinna Mustafa; Ammar A. Mohammed; Yousif Ali; Maha M. Osman; Mohamed S. Ali; Rihab A. Omer; Ayman Ahmed; Carol H. Sibley

doi:10.1186/s13104-020-05363-0

A snapshot of Plasmodium falciparum malaria drug resistance markers in Sudan: a pilot study

Nouh S. Mohamed^*, Hanadi Abdelbagi, Hussam A. Osman, Abdallah E. Ahmed, Alaa M. Yousif, Yusraa B. Edris, Eman Y. Osman, Aahd R. Elsadig, Emmanuel E. Siddig, Madinna Mustafa, Ammar A. Mohammed, Yousif Ali, Maha M. Osman, Mohamed S. Ali, Rihab A. Omer, Ayman Ahmed, Carol H. Sibley

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

7 Citations (Scopus)

Abstract

Objectives: Malaria infection is still known to be a worldwide public health problem, especially in tropical and sub-tropical African countries like Sudan. A pilot study conducted to describe the trend of P. falciparum drug resistance markers in 2017–2018 in comparison to CQ and AS/SP eras in Sudan. The Pfcrt, Pfmdr-1, Pfdhfr, and Pfdhps genes were investigated. Data deposited by the worldwide antimalarial resistance network was consulted, and the molecular markers previously reported from Sudan were analyzed. Results: Drug molecular markers analysis was successfully done on 20 P. falciparum isolates. The Pfcrt K76 showed high frequency; 16 (80%). For the Pfmdr-1, 9 (45%) isolates were carrying the N86 allele, and 11 (55%) were 86Y allele. While the Y184F of the Pfmdr-1 showed a higher frequency of 184F compared to Y184; 16 (80%) and 4 (20%), respectively. In the Pfdhfr, 51I allele showed higher frequency compared to N51; 18 (90%) and 2 (10%), respectively. For S108N, 18 (90%) were 108 N and 2 (10%) were S108. In the Pfdhps, all isolates were carrying the mutant alleles; 437G and 540E. The frequency distribution of the Pfcrt, Pfmdr-1, Pfdhfr, Pfdhps was significantly different across the whole years in Sudan.

Original language	English
Article number	512
Journal	BMC Research Notes
Volume	13
Issue number	1
DOIs	https://doi.org/10.1186/s13104-020-05363-0
Publication status	Published - 1 Dec 2020
Externally published	Yes

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Publisher Copyright:
© 2020, The Author(s).

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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Mohamed, N. S., Abdelbagi, H., Osman, H. A., Ahmed, A. E., Yousif, A. M., Edris, Y. B., Osman, E. Y., Elsadig, A. R., Siddig, E. E., Mustafa, M., Mohammed, A. A., Ali, Y., Osman, M. M., Ali, M. S., Omer, R. A., Ahmed, A., & Sibley, C. H. (2020). A snapshot of Plasmodium falciparum malaria drug resistance markers in Sudan: a pilot study. BMC Research Notes, 13(1), Article 512. https://doi.org/10.1186/s13104-020-05363-0

@article{14207430c9ab440baa91122d2a096b20,

title = "A snapshot of Plasmodium falciparum malaria drug resistance markers in Sudan: a pilot study",

abstract = "Objectives: Malaria infection is still known to be a worldwide public health problem, especially in tropical and sub-tropical African countries like Sudan. A pilot study conducted to describe the trend of P. falciparum drug resistance markers in 2017–2018 in comparison to CQ and AS/SP eras in Sudan. The Pfcrt, Pfmdr-1, Pfdhfr, and Pfdhps genes were investigated. Data deposited by the worldwide antimalarial resistance network was consulted, and the molecular markers previously reported from Sudan were analyzed. Results: Drug molecular markers analysis was successfully done on 20 P. falciparum isolates. The Pfcrt K76 showed high frequency; 16 (80%). For the Pfmdr-1, 9 (45%) isolates were carrying the N86 allele, and 11 (55%) were 86Y allele. While the Y184F of the Pfmdr-1 showed a higher frequency of 184F compared to Y184; 16 (80%) and 4 (20%), respectively. In the Pfdhfr, 51I allele showed higher frequency compared to N51; 18 (90%) and 2 (10%), respectively. For S108N, 18 (90%) were 108 N and 2 (10%) were S108. In the Pfdhps, all isolates were carrying the mutant alleles; 437G and 540E. The frequency distribution of the Pfcrt, Pfmdr-1, Pfdhfr, Pfdhps was significantly different across the whole years in Sudan.",

author = "Mohamed, {Nouh S.} and Hanadi Abdelbagi and Osman, {Hussam A.} and Ahmed, {Abdallah E.} and Yousif, {Alaa M.} and Edris, {Yusraa B.} and Osman, {Eman Y.} and Elsadig, {Aahd R.} and Siddig, {Emmanuel E.} and Madinna Mustafa and Mohammed, {Ammar A.} and Yousif Ali and Osman, {Maha M.} and Ali, {Mohamed S.} and Omer, {Rihab A.} and Ayman Ahmed and Sibley, {Carol H.}",

note = "Publisher Copyright: {\textcopyright} 2020, The Author(s).",

year = "2020",

month = dec,

day = "1",

doi = "10.1186/s13104-020-05363-0",

language = "English",

volume = "13",

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Mohamed, NS, Abdelbagi, H, Osman, HA, Ahmed, AE, Yousif, AM, Edris, YB, Osman, EY, Elsadig, AR, Siddig, EE, Mustafa, M, Mohammed, AA, Ali, Y, Osman, MM, Ali, MS, Omer, RA, Ahmed, A & Sibley, CH 2020, 'A snapshot of Plasmodium falciparum malaria drug resistance markers in Sudan: a pilot study', BMC Research Notes, vol. 13, no. 1, 512. https://doi.org/10.1186/s13104-020-05363-0

TY - JOUR

T1 - A snapshot of Plasmodium falciparum malaria drug resistance markers in Sudan

T2 - a pilot study

AU - Mohamed, Nouh S.

AU - Abdelbagi, Hanadi

AU - Osman, Hussam A.

AU - Ahmed, Abdallah E.

AU - Yousif, Alaa M.

AU - Edris, Yusraa B.

AU - Osman, Eman Y.

AU - Elsadig, Aahd R.

AU - Siddig, Emmanuel E.

AU - Mustafa, Madinna

AU - Mohammed, Ammar A.

AU - Ali, Yousif

AU - Osman, Maha M.

AU - Ali, Mohamed S.

AU - Omer, Rihab A.

AU - Ahmed, Ayman

AU - Sibley, Carol H.

PY - 2020/12/1

Y1 - 2020/12/1

N2 - Objectives: Malaria infection is still known to be a worldwide public health problem, especially in tropical and sub-tropical African countries like Sudan. A pilot study conducted to describe the trend of P. falciparum drug resistance markers in 2017–2018 in comparison to CQ and AS/SP eras in Sudan. The Pfcrt, Pfmdr-1, Pfdhfr, and Pfdhps genes were investigated. Data deposited by the worldwide antimalarial resistance network was consulted, and the molecular markers previously reported from Sudan were analyzed. Results: Drug molecular markers analysis was successfully done on 20 P. falciparum isolates. The Pfcrt K76 showed high frequency; 16 (80%). For the Pfmdr-1, 9 (45%) isolates were carrying the N86 allele, and 11 (55%) were 86Y allele. While the Y184F of the Pfmdr-1 showed a higher frequency of 184F compared to Y184; 16 (80%) and 4 (20%), respectively. In the Pfdhfr, 51I allele showed higher frequency compared to N51; 18 (90%) and 2 (10%), respectively. For S108N, 18 (90%) were 108 N and 2 (10%) were S108. In the Pfdhps, all isolates were carrying the mutant alleles; 437G and 540E. The frequency distribution of the Pfcrt, Pfmdr-1, Pfdhfr, Pfdhps was significantly different across the whole years in Sudan.

AB - Objectives: Malaria infection is still known to be a worldwide public health problem, especially in tropical and sub-tropical African countries like Sudan. A pilot study conducted to describe the trend of P. falciparum drug resistance markers in 2017–2018 in comparison to CQ and AS/SP eras in Sudan. The Pfcrt, Pfmdr-1, Pfdhfr, and Pfdhps genes were investigated. Data deposited by the worldwide antimalarial resistance network was consulted, and the molecular markers previously reported from Sudan were analyzed. Results: Drug molecular markers analysis was successfully done on 20 P. falciparum isolates. The Pfcrt K76 showed high frequency; 16 (80%). For the Pfmdr-1, 9 (45%) isolates were carrying the N86 allele, and 11 (55%) were 86Y allele. While the Y184F of the Pfmdr-1 showed a higher frequency of 184F compared to Y184; 16 (80%) and 4 (20%), respectively. In the Pfdhfr, 51I allele showed higher frequency compared to N51; 18 (90%) and 2 (10%), respectively. For S108N, 18 (90%) were 108 N and 2 (10%) were S108. In the Pfdhps, all isolates were carrying the mutant alleles; 437G and 540E. The frequency distribution of the Pfcrt, Pfmdr-1, Pfdhfr, Pfdhps was significantly different across the whole years in Sudan.

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DO - 10.1186/s13104-020-05363-0

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SN - 1756-0500

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JO - BMC Research Notes

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ER -

A snapshot of Plasmodium falciparum malaria drug resistance markers in Sudan: a pilot study

Abstract

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