A spontaneously in vitro transformed mesothelial cell line has a similar pattern of PDGF chain and PDGF receptor expression to malignant mesothelioma cell lines

A. W. Langerak*, H. Vietsch, M. J. Bouts, A. Hagemeijer, M. A. Versnel

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

6 Citations (Scopus)

Abstract

The cell line TM-2 was obtained by spontaneous in vitro transformation of the normal mesothelial cell line NM-7. In contrast to NM-7, TM-2 was found to have an abnormal karyotype and an indefinite lifespan, and to proliferate independently of the addition of growth factors to the culture medium. TM-2 showed platelet-derived growth factor (PDGF) A-chain, B-chain and β-receptor messenger ribonucleic acid (mRNA) expression, whereas NM-7 expressed PDGF A-chain, α-receptor and β-receptor mRNA. PDGF α- and β-receptor protein expression in TM-2 and NM-7 confirmed the results obtained by Northern blot analysis. The expression pattern of PDGF chains and receptors in TM-2 strongly resembled the pattern found in malignant mesothelioma cell lines. These results support the hypothesis that PDGF-BB is an autocrine growth factor in malignant mesothelioma cell lines and plays a role in the transformation of normal mesothelial cells.

Original languageEnglish
Pages (from-to)170-174
Number of pages5
JournalEuropean Respiratory Review
Volume3
Issue number11
Publication statusPublished - 1993

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