A systems approach to analyze transcription factors in mammalian cells

Eric Soler; C Andrieu-Soler; Ernie Boer; JC Bryne; S Thongjuea; E Rijkers; Jeroen Demmers; Wilfred van Ijcken; Frank Grosveld

doi:10.1016/j.ymeth.2010.08.002

A systems approach to analyze transcription factors in mammalian cells

Eric Soler, C Andrieu-Soler, Ernie Boer, JC Bryne, S Thongjuea, E Rijkers, Jeroen Demmers, Wilfred van Ijcken, Frank Grosveld

Research output: Contribution to journal › Article › Academic › peer-review

19 Citations (Scopus)

Abstract

Transcription factors (TFs) play a central role in the development of multicellular organisms. The sequential actions of critical TFs direct cells to adopt defined differentiation pathways leading to functional, fully differentiated tissues. Here, we describe a generic experimental pipeline that integrates biochemistry, genetics and next generation sequencing with bioinformatics to characterize TF complexes composition, function and target genes at a genome-wide scale. We show an application of this experimental pipeline which aims to unravel the molecular events taking place during hematopoietic cell differentiation. (C) 2010 Elsevier Inc. All rights reserved.

Original language	Undefined/Unknown
Pages (from-to)	151-162
Number of pages	12
Journal	Methods-A Companion to Methods in Enzymology
Volume	53
Issue number	2
DOIs	https://doi.org/10.1016/j.ymeth.2010.08.002
Publication status	Published - 2011

Research programs

EMC MGC-02-13-02
EMC MGC-02-21-01

Access to Document

10.1016/j.ymeth.2010.08.002

http://hdl.handle.net/1765/31698

Cite this

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AU - Andrieu-Soler, C

AU - Boer, Ernie

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AU - Thongjuea, S

AU - Rijkers, E

AU - Demmers, Jeroen

AU - van Ijcken, Wilfred

AU - Grosveld, Frank

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