Activity of cerebellar nuclei neurons correlates with zebrinii identity of their purkinje cell afferents

Gerrit C. Beekhof, Simona V. Gornati, Cathrin B. Canto, Avraham M. Libster, Martijn Schonewille*, Chris I. De Zeeuw, Freek E. Hoebeek

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

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Abstract

Purkinje cells (PCs) in the cerebellar cortex can be divided into at least two main subpop-ulations: one subpopulation that prominently expresses ZebrinII (Z+), and shows a relatively low simple spike firing rate, and another that hardly expresses ZebrinII (Z–) and shows higher baseline firing rates. Likewise, the complex spike responses of PCs, which are evoked by climbing fiber inputs and thus reflect the activity of the inferior olive (IO), show the same dichotomy. However, it is not known whether the target neurons of PCs in the cerebellar nuclei (CN) maintain this bimodal distribution. Electrophysiological recordings in awake adult mice show that the rate of action potential firing of CN neurons that receive input from Z+ PCs was consistently lower than that of CN neurons innervated by Z– PCs. Similar in vivo recordings in juvenile and adolescent mice indicated that the firing frequency of CN neurons correlates to the ZebrinII identity of the PC afferents in adult, but not postnatal stages. Finally, the spontaneous action potential firing pattern of adult CN neurons recorded in vitro revealed no significant differences in intrinsic pacemaking activity between ZebrinII identities. Our findings indicate that all three main components of the olivocerebel-lar loop, i.e., PCs, IO neurons and CN neurons, operate at a higher rate in the Z– modules.

Original languageEnglish
Article number2686
JournalCells
Volume10
Issue number10
DOIs
Publication statusPublished - 7 Oct 2021

Bibliographical note

Funding Information:
Funding: S.V.G. and F.E.H. are supported by the Dutch organization for life sciences (NWO‐ALW; VIDI grant #016.121.346), Medical Sciences (TOP‐GO #91210067) and the C.J. Vaillant fund. M.S. is supported by the European Research Council Starting Grant (ERC‐Stg; #680235). C.I.D.Z. is sup‐ ported by the Dutch Organization for Medical Sciences (Zon‐MW; TOP‐GO #91210067), Life Sci‐ ences (ALW; #854.10.004), ERC‐adv (#294775), ERC‐POC (#768914) and LISTEN (#6) of the EU, as well as Medical NeuroDelta (#7) and INTENSE of LSH‐NWO (#8), Albinism Vriendenfonds NIN, van Raamsdonk fonds, and the Trustfonds Rotterdam for support. C.B.C is supported by NWO‐ ALW (VENI grant # 863.14.005). None of the funding bodies had any input to the study design or outcome.

Funding Information:
S.V.G. and F.E.H. are supported by the Dutch organization for life sciences (NWO?ALW; VIDI grant #016.121.346), Medical Sciences (TOP?GO #91210067) and the C.J. Vaillant fund. M.S. is supported by the European Research Council Starting Grant (ERC?Stg; #680235). C.I.D.Z. is supported by the Dutch Organization for Medical Sciences (Zon?MW; TOP?GO #91210067), Life Sciences (ALW; #854.10.004), ERC?adv (#294775), ERC?POC (#768914) and LISTEN (#6) of the EU, as well as Medical NeuroDelta (#7) and INTENSE of LSH?NWO (#8), Albinism Vriendenfonds NIN, van Raamsdonk fonds, and the Trustfonds Rotterdam for support. C.B.C is supported by NWO? ALW (VENI grant # 863.14.005). None of the funding bodies had any input to the study design or outcome.

Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.

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