Acute but transient release of terminal complement complex after reperfusion in clinical kidney transplantation

Dorottya K. De Vries*, Pieter Van Der Pol, Gerritje E. Van Anken, Danielle J. Van Gijlswijk, Jeffrey Damman, Jan H. Lindeman, Marlies E.J. Reinders, Alexander F. Schaapherder, Cees Van Kooten

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

65 Citations (Scopus)

Abstract

Background. Ischemia/reperfusion (I/R) injury has a major impact on kidney graft function and survival. Animal studies have suggested a role for complement activation in mediating I/R injury; however, results are not unambiguous. Whether complement activation is involved in clinical I/R injury in humans is still unclear. Methods. In the present study, we assessed the formation and release of C5b-9 during early reperfusion in clinical kidney transplantation in living donor, brain-dead donor, and cardiac dead donor kidney transplantation. By arteriovenous measurements and histologic studies, local terminal complement activation in the reperfused kidney was assessed. Results. There was no release of soluble C5b-9 (sC5b-9) from living donor kidneys, nor was there a release of C5a. In contrast, instantly after reperfusion, there was a significant but transient venous release of sC5b-9 from the reperfused kidney graft in brain-dead donor and cardiac dead donor kidney transplantation. This short-term activation of the terminal complement cascade in deceased-donor kidney transplantation was not reflected by renal tissue deposition of C5b-9 in biopsies taken 45 min after reperfusion. Conclusions. This systematic study in human kidney transplantation shows an acute but nonsustained sC5b-9 release on reperfusion in deceased-donor kidney transplantation. This instantaneous, intravascular terminal complement activation may be induced by intravascular cellular debris and hypoxic or injured endothelium.

Original languageEnglish
Pages (from-to)816-820
Number of pages5
JournalTransplantation
Volume95
Issue number6
DOIs
Publication statusPublished - 27 May 2013
Externally publishedYes

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