@inproceedings{55a7c87a0cbc4fadb2993a7103f218df,
title = "Adaptive optics for confocal laser scanning microscopy with adjustable pinhole",
abstract = "The pinhole plays an important role in confocal laser scanning microscopy (CLSM) for adaptive optics (AO) as well as in imaging, where the size of the pinhole denotes a trade-off between out-of-focus rejection and wavefront distortion. This contribution proposes an AO system for a commercial CLSM with an adjustable square pinhole to cope with such a trade-off. The proposed adjustable pinhole enables to calibrate the AO system and to evaluate the imaging performance. Experimental results with fluorescence beads on the coverslip and at a depth of 40 μm in the human hepatocellular carcinoma cell spheroid demonstrate that the proposed AO system can improve the image quality by the proposed calibration method. The proposed pinhole intensity ratio also indicates the image improvement by the AO correction in intensity as well as resolution.",
author = "Yoo, {Han Woong} and {Van Royen}, {Martin E.} and {Van Cappellen}, {Wiggert A.} and Houtsmuller, {Adriaan B.} and Michel Verhaegen and Georg Schitter",
note = "Publisher Copyright: {\textcopyright} 2016 SPIE.; Biophotonics: Photonic Solutions for Better Health Care V ; Conference date: 04-04-2016 Through 07-04-2016",
year = "2016",
doi = "10.1117/12.2227775",
language = "English",
series = "Proceedings of SPIE - The International Society for Optical Engineering",
publisher = "SPIE",
editor = "Tuchin, {Valery V.} and Pavone, {Francesco S.} and Jurgen Popp and Matthews, {Dennis L.}",
booktitle = "Biophotonics",
address = "United States",
}