Adaptive optics for confocal laser scanning microscopy with adjustable pinhole

Han Woong Yoo*, Martin E. Van Royen, Wiggert A. Van Cappellen, Adriaan B. Houtsmuller, Michel Verhaegen, Georg Schitter

*Corresponding author for this work

Research output: Chapter/Conference proceedingConference proceedingAcademicpeer-review

3 Citations (Scopus)

Abstract

The pinhole plays an important role in confocal laser scanning microscopy (CLSM) for adaptive optics (AO) as well as in imaging, where the size of the pinhole denotes a trade-off between out-of-focus rejection and wavefront distortion. This contribution proposes an AO system for a commercial CLSM with an adjustable square pinhole to cope with such a trade-off. The proposed adjustable pinhole enables to calibrate the AO system and to evaluate the imaging performance. Experimental results with fluorescence beads on the coverslip and at a depth of 40 μm in the human hepatocellular carcinoma cell spheroid demonstrate that the proposed AO system can improve the image quality by the proposed calibration method. The proposed pinhole intensity ratio also indicates the image improvement by the AO correction in intensity as well as resolution.

Original languageEnglish
Title of host publicationBiophotonics
Subtitle of host publicationPhotonic Solutions for Better Health Care V
EditorsValery V. Tuchin, Francesco S. Pavone, Jurgen Popp, Dennis L. Matthews
PublisherSPIE
ISBN (Electronic)9781510601321
DOIs
Publication statusPublished - 2016
EventBiophotonics: Photonic Solutions for Better Health Care V - Brussels, Belgium
Duration: 4 Apr 20167 Apr 2016

Publication series

SeriesProceedings of SPIE - The International Society for Optical Engineering
Volume9887
ISSN0277-786X

Conference

ConferenceBiophotonics: Photonic Solutions for Better Health Care V
Country/TerritoryBelgium
CityBrussels
Period4/04/167/04/16

Bibliographical note

Publisher Copyright: © 2016 SPIE.

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