Adenosine Deaminase Acts as a Natural Antagonist for Dipeptidyl Peptidase 4-Mediated Entry of the Middle East Respiratory Syndrome Coronavirus

Stalinraj Victor, Saskia Smits, LB Provacia, Judith van den Brand, L Wiersma, Werner Ouwendijk, Theo Bestebroer, Monique Spronken, Geert Amerongen, PJM Rottier, Ron Fouchier, BJ Bosch, Ab Osterhaus, Bart Haagmans

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Abstract

Middle East respiratory syndrome coronavirus (MERS-CoV) replicates in cells of different species using dipeptidyl peptidase 4 (DPP4) as a functional receptor. Here we show the resistance of ferrets to MERS-CoV infection and inability of ferret DDP4 to bind MERS-CoV. Site-directed mutagenesis of amino acids variable in ferret DPP4 thus revealed the functional human DPP4 virus binding site. Adenosine deaminase (ADA), a DPP4 binding protein, competed for virus binding, acting as a natural antagonist for MERS-CoV infection.
Original languageUndefined/Unknown
Pages (from-to)1834-1838
Number of pages5
JournalJournal of Virology
Volume88
Issue number3
DOIs
Publication statusPublished - 2014

Research programs

  • EMC MM-04-27-01

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