Age-related curves of AMH using the Gen II, the picoAMH and the Elecsys assays in women with polycystic ovary syndrome

Federica Barbagallo*, Kim van der Ham, Sten P Willemsen, Yvonne V Louwers, Joop S Laven

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

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Abstract

CONTEXT: 

Several challenges still exist to adopt the anti-Müllerian hormone (AMH) as a marker of polycystic ovary morphology (PCOM), as included in the recently updated international guideline. Although different evaluations of age- and assay-specific reference ranges have been published in the last years, these studies have mainly been conducted in normo-ovulatory or infertile women.

OBJECTIVE: 

To develop an age-specific percentile distribution of AMH in patients with polycystic ovary syndrome (PCOS) measured by three different assays.

DESIGN: 

Retrospective cross-sectional study.

PATIENTS: 

2,725 women aged 20 to 40 years with PCOS diagnosis were included.

INTERVENTION (S): 

Serum AMH measurement by the Gen II (Beckman Coulter), the picoAMH (Ansh Labs), and the Elecsys (Roche) assays.

MAIN OUTCOME MEAUSRE (S): 

Age-specific centile curves for all the assays and correlations between AMH, clinical, hormonal, and ultrasound characteristics.

RESULTS: 

Age-related nomograms for the 5th, 10th, 25th, 50th, 75th, 90th, and 95th percentiles of AMH were calculated using the LMS method for all the assays. AMH levels were significantly different between PCOS phenotypes. AMH levels were positive correlated to luteinizing hormone (LH), LH/follicular stimulating hormone (FSH) ratio, testosterone, androstenedione, free androgen index, mean follicular number, and mean ovarian volume.

CONCLUSIONS: 

To our knowledge this is the first study reporting age specific percentile nomograms of serum AMH levels measured by the Gen II, the picoAMH and the Elecsys assays in a large population of PCOS women. These findings may help to interpret AMH levels in PCOS patients and facilitate the use of AMH as a diagnostic tool across age ranges.

Original languageEnglish
JournalThe Journal of clinical endocrinology and metabolism
DOIs
Publication statusE-pub ahead of print - 15 Mar 2024

Bibliographical note

© The Author(s) 2024. Published by Oxford University Press on behalf of the Endocrine Society.

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