Abstract
Isolated myofibers offer the possibility of in vitro study of satellite cells in their niche. We describe a mouse myofiber isolation assay to assess satellite cell activation by quantifying myofiber-derived satellite cell progeny. The assay allows isolation of myofibers from a mouse using standard equipment and reagents. It can be used to compare satellite cells across different mouse models or to evaluate their response to treatments, offering a valuable complementary tool for in vitro experimentation.
| Original language | English |
|---|---|
| Article number | 100482 |
| Journal | STAR Protocols |
| Volume | 2 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 18 Jun 2021 |
Bibliographical note
Funding Information:The work is funded through the Center for Lysosomal and Metabolic Diseases at Erasmus MC University Medical Center and the Prinses Beatrix Spierfonds /Stichting Spieren voor Spieren (Project number W.OR13–21). Imaging was performed at the Erasmus MC Optical Imaging Center.
Funding Information:
The work is funded through the Center for Lysosomal and Metabolic Diseases at Erasmus MC University Medical Center and the Prinses Beatrix Spierfonds/Stichting Spieren voor Spieren (Project number W.OR13?21). Imaging was performed at the Erasmus MC Optical Imaging Center. Conceptualization, R.C.F. P.P. and G.S.; investigation, R.C.F. E.B. S.B. and G.S.; writing and editing, R.C.F. P.P. and G.S.; funding acquisition, P.P. and G.S. The authors declare no competing interests.
Publisher Copyright:
© 2021 The Author(s)