An in vitro assay to quantify satellite cell activation using isolated mouse myofibers

Rodrigo Canibano-Fraile; Emma Boertjes; Stela Bozhilova; W. W.M.Pim Pijnappel; Gerben J. Schaaf

doi:10.1016/j.xpro.2021.100482

An in vitro assay to quantify satellite cell activation using isolated mouse myofibers

Rodrigo Canibano-Fraile
, Emma Boertjes
, Stela Bozhilova
, W. W.M.Pim Pijnappel
, Gerben J. Schaaf^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

1 Citation (Scopus)

Abstract

Isolated myofibers offer the possibility of in vitro study of satellite cells in their niche. We describe a mouse myofiber isolation assay to assess satellite cell activation by quantifying myofiber-derived satellite cell progeny. The assay allows isolation of myofibers from a mouse using standard equipment and reagents. It can be used to compare satellite cells across different mouse models or to evaluate their response to treatments, offering a valuable complementary tool for in vitro experimentation.

Original language	English
Article number	100482
Journal	STAR Protocols
Volume	2
Issue number	2
DOIs	https://doi.org/10.1016/j.xpro.2021.100482
Publication status	Published - 18 Jun 2021

Bibliographical note

Funding Information:
The work is funded through the Center for Lysosomal and Metabolic Diseases at Erasmus MC University Medical Center and the Prinses Beatrix Spierfonds /Stichting Spieren voor Spieren (Project number W.OR13–21). Imaging was performed at the Erasmus MC Optical Imaging Center.

Funding Information:
The work is funded through the Center for Lysosomal and Metabolic Diseases at Erasmus MC University Medical Center and the Prinses Beatrix Spierfonds/Stichting Spieren voor Spieren (Project number W.OR13?21). Imaging was performed at the Erasmus MC Optical Imaging Center. Conceptualization, R.C.F. P.P. and G.S.; investigation, R.C.F. E.B. S.B. and G.S.; writing and editing, R.C.F. P.P. and G.S.; funding acquisition, P.P. and G.S. The authors declare no competing interests.

Publisher Copyright:
© 2021 The Author(s)

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10.1016/j.xpro.2021.100482

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title = "An in vitro assay to quantify satellite cell activation using isolated mouse myofibers",

abstract = "Isolated myofibers offer the possibility of in vitro study of satellite cells in their niche. We describe a mouse myofiber isolation assay to assess satellite cell activation by quantifying myofiber-derived satellite cell progeny. The assay allows isolation of myofibers from a mouse using standard equipment and reagents. It can be used to compare satellite cells across different mouse models or to evaluate their response to treatments, offering a valuable complementary tool for in vitro experimentation.",

author = "Rodrigo Canibano-Fraile and Emma Boertjes and Stela Bozhilova and Pijnappel, \{W. W.M.Pim\} and Schaaf, \{Gerben J.\}",

note = "Funding Information: The work is funded through the Center for Lysosomal and Metabolic Diseases at Erasmus MC University Medical Center and the Prinses Beatrix Spierfonds /Stichting Spieren voor Spieren (Project number W.OR13–21). Imaging was performed at the Erasmus MC Optical Imaging Center. Funding Information: The work is funded through the Center for Lysosomal and Metabolic Diseases at Erasmus MC University Medical Center and the Prinses Beatrix Spierfonds/Stichting Spieren voor Spieren (Project number W.OR13?21). Imaging was performed at the Erasmus MC Optical Imaging Center. Conceptualization, R.C.F. P.P. and G.S.; investigation, R.C.F. E.B. S.B. and G.S.; writing and editing, R.C.F. P.P. and G.S.; funding acquisition, P.P. and G.S. The authors declare no competing interests. Publisher Copyright: {\textcopyright} 2021 The Author(s)",

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AU - Boertjes, Emma

AU - Bozhilova, Stela

AU - Pijnappel, W. W.M.Pim

AU - Schaaf, Gerben J.

N1 - Funding Information: The work is funded through the Center for Lysosomal and Metabolic Diseases at Erasmus MC University Medical Center and the Prinses Beatrix Spierfonds /Stichting Spieren voor Spieren (Project number W.OR13–21). Imaging was performed at the Erasmus MC Optical Imaging Center. Funding Information: The work is funded through the Center for Lysosomal and Metabolic Diseases at Erasmus MC University Medical Center and the Prinses Beatrix Spierfonds/Stichting Spieren voor Spieren (Project number W.OR13?21). Imaging was performed at the Erasmus MC Optical Imaging Center. Conceptualization, R.C.F. P.P. and G.S.; investigation, R.C.F. E.B. S.B. and G.S.; writing and editing, R.C.F. P.P. and G.S.; funding acquisition, P.P. and G.S. The authors declare no competing interests. Publisher Copyright: © 2021 The Author(s)

PY - 2021/6/18

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N2 - Isolated myofibers offer the possibility of in vitro study of satellite cells in their niche. We describe a mouse myofiber isolation assay to assess satellite cell activation by quantifying myofiber-derived satellite cell progeny. The assay allows isolation of myofibers from a mouse using standard equipment and reagents. It can be used to compare satellite cells across different mouse models or to evaluate their response to treatments, offering a valuable complementary tool for in vitro experimentation.

AB - Isolated myofibers offer the possibility of in vitro study of satellite cells in their niche. We describe a mouse myofiber isolation assay to assess satellite cell activation by quantifying myofiber-derived satellite cell progeny. The assay allows isolation of myofibers from a mouse using standard equipment and reagents. It can be used to compare satellite cells across different mouse models or to evaluate their response to treatments, offering a valuable complementary tool for in vitro experimentation.

UR - https://www.scopus.com/pages/publications/85104661240

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DO - 10.1016/j.xpro.2021.100482

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An in vitro assay to quantify satellite cell activation using isolated mouse myofibers

Abstract

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