TY - JOUR
T1 - An optimized enzyme-linked lectin assay to measure influenza A virus neuraminidase inhibition antibody titers in human sera
AU - Couzens, Laura
AU - Gao, Jin
AU - Westgeest, Kim
AU - Sandbulte, Matthew
AU - Lugovtsev, Vladimir
AU - Fouchier, Ron
AU - Eichelberger, Maryna
PY - 2014/12/15
Y1 - 2014/12/15
N2 - Antibodies to neuraminidase (NA), the second most abundant surface protein on influenza virus, contribute toward protection against influenza. The traditional thiobarbituric acid (TBA) method to quantify NA inhibiting antibodies is cumbersome and not suitable for routine serology. An enzyme-linked lectin assay (ELLA) described by Lambre et al. (1990) is a practical alternative method for measuring NA inhibition (NI) titers. This report describes optimization of the ELLA for measuring NI titers in human sera against influenza A viruses, using H6N1 and H6N2 viruses as antigens. The optimized ELLA is subtype-specific and reproducible. While the titers measured by ELLA are somewhat greater than those measured by a miniaturized TBA method, seroconversion rates are the same, suggesting similarity in assay sensitivity under these optimized conditions. The ELLA described in this report provides a practical format for routine evaluation of human antibody responses to NA.
AB - Antibodies to neuraminidase (NA), the second most abundant surface protein on influenza virus, contribute toward protection against influenza. The traditional thiobarbituric acid (TBA) method to quantify NA inhibiting antibodies is cumbersome and not suitable for routine serology. An enzyme-linked lectin assay (ELLA) described by Lambre et al. (1990) is a practical alternative method for measuring NA inhibition (NI) titers. This report describes optimization of the ELLA for measuring NI titers in human sera against influenza A viruses, using H6N1 and H6N2 viruses as antigens. The optimized ELLA is subtype-specific and reproducible. While the titers measured by ELLA are somewhat greater than those measured by a miniaturized TBA method, seroconversion rates are the same, suggesting similarity in assay sensitivity under these optimized conditions. The ELLA described in this report provides a practical format for routine evaluation of human antibody responses to NA.
U2 - 10.1016/j.jviromet.2014.09.003
DO - 10.1016/j.jviromet.2014.09.003
M3 - Article
C2 - 25233882
SN - 0166-0934
VL - 210
SP - 7
EP - 14
JO - Journal of Virological Methods
JF - Journal of Virological Methods
ER -