Association of muscular strength and targeted proteomics involved in brain health in children with overweight/obesity

Marcos Olvera-Rojas; Abel Plaza-Florido; Patricio Solis-Urra; María Rodriguez-Ayllon; Angel Toval; Irene Esteban-Cornejo; Francisco B. Ortega

doi:10.1111/sms.14387

Association of muscular strength and targeted proteomics involved in brain health in children with overweight/obesity

Marcos Olvera-Rojas^*
, Abel Plaza-Florido
, Patricio Solis-Urra
, María Rodriguez-Ayllon
, Angel Toval
, Irene Esteban-Cornejo
, Francisco B. Ortega^*

^*Corresponding author for this work

University of Granada
University of California at Irvine
Universidad Andrés Bello
Hospital Universitario Virgen de las Nieves
Erasmus University Rotterdam
Centro de Investigación Biomédica en Red (CIBER)
University of Jyväskylä

Research output: Contribution to journal › Article › Academic › peer-review

6 Citations (Scopus)

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Abstract

Muscular strength has been positively associated with better brain health indicators during childhood obesity. However, the molecular mechanisms underlying the positive impact of muscular strength in brain health are poorly understood. We aimed to study the association of muscular strength with neurology-related circulating proteins in plasma in children with overweight/obesity and to explore the role of cardiorespiratory fitness (CRF) as a confounder. The participants were 86 Caucasian children (10.1 ± 1.1 years old; 41% girls) from the ActiveBrains project. Muscular strength was measured by field and laboratory tests. CRF was assessed with an incremental treadmill test. Olink's technology was used to quantify 92 neurology-related proteins in plasma. Protein–protein interactions were computed using the STRING website. Muscular strength was positively associated with 12 proteins (BetaNGF, CDH6, CLEC10A, CLM1, FcRL2, HAGH, IL12, LAIR2, MSR1, SCARB2, SMOC2, and TNFRSF12A), and negatively associated with 12 proteins (CLEC1B, CTSC, CTSS, gal-8, GCP5, NAAA, NrCAM, NTRK2, PLXNB3, RSPO1, sFRP3, and THY1). After adjustment for CRF, muscular strength was positively associated with eight proteins (BetaNGF, CDH6, CLEC10A, FcRL2, LAIR2, MSR1, SCARB2, and TNFRSF12A) and negatively associated with two proteins (gal-8 and NrCAM). After applying FDR correction, only CLEC10A remained statistically significant. In conclusion, muscular strength was associated with blood circulating proteins involved in several biological processes, particularly anti-inflammatory response, lipid metabolism, beta amyloid clearance, and neuronal action potential propagation. More powered studies are warranted in pediatric populations to contrast or confirm our findings.

Original language	English
Pages (from-to)	1738-1751
Number of pages	14
Journal	Scandinavian Journal of Medicine and Science in Sports
Volume	33
Issue number	9
Early online date	15 May 2023
DOIs	https://doi.org/10.1111/sms.14387
Publication status	Published - Sept 2023
Externally published	Yes

Bibliographical note

Funding Information:
The present study was supported by grants from the Spanish Ministry of Economy and Competitiveness (DEP2013‐47540, DEP2016‐79512‐R, and DEP2017‐91544‐EXP), European Regional Development Fund (ERDF) of the European Commission (Number 667302), and by the Alicia Koplowitz Foundation. Supplementary funding was obtained from the Andalusian Operational Programme supported with ERDF (FEDER in Spanish, B‐CTS‐355‐UGR18). This study was also supported by the University of Granada, Plan Propio de Investigación, Visiting Scholar grants and Excellence actions: Units of Excellence; Unit of Excellence on Exercise, Nutrition and Health (UCEENS) and by the Junta de Andalucía, Consejería de Conocimiento, Investigación y Universidades and the ERDF (SOMM17/6107/UGR). The present study was further supported by the EXERNET Research Network on Exercise and Health (DEP2005‐00046/ACTI) and by the High Council of Sports (09/UPB/19). AP‐F contribution was funded in part by NIH grant #: U01TR002004 (project REACH). MR‐A was supported by the Alicia Koplowitz Foundation. IE‐C is supported by the Spanish Ministry of Science and Innovation (RyC2019‐027287‐1). PS‐U is supported by a grant from ANID/BECAS Chile/72180543 and through a Margarita Salas grant from the Spanish Ministry Universities. AT is supported by the Junta de Andalucía postdoctoral research grant. Funding for open access charge: Universidad de Granada / CBUA. This work is part of Ph.D. Thesis conducted in the Biomedicine Doctoral Studies of the University of Granada, Spain.

Publisher Copyright:
© 2023 The Authors. Scandinavian Journal of Medicine & Science In Sports published by John Wiley & Sons Ltd.

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Association of muscular strength and targeted proteomics involved in brain health in children with overweight obesityFinal published version, 1.03 MBLicence: CC BY

Cite this

@article{9a5d5ed974c943dba88c0fb9b26f9566,

title = "Association of muscular strength and targeted proteomics involved in brain health in children with overweight/obesity",

abstract = "Muscular strength has been positively associated with better brain health indicators during childhood obesity. However, the molecular mechanisms underlying the positive impact of muscular strength in brain health are poorly understood. We aimed to study the association of muscular strength with neurology-related circulating proteins in plasma in children with overweight/obesity and to explore the role of cardiorespiratory fitness (CRF) as a confounder. The participants were 86 Caucasian children (10.1 ± 1.1 years old; 41\% girls) from the ActiveBrains project. Muscular strength was measured by field and laboratory tests. CRF was assessed with an incremental treadmill test. Olink's technology was used to quantify 92 neurology-related proteins in plasma. Protein–protein interactions were computed using the STRING website. Muscular strength was positively associated with 12 proteins (BetaNGF, CDH6, CLEC10A, CLM1, FcRL2, HAGH, IL12, LAIR2, MSR1, SCARB2, SMOC2, and TNFRSF12A), and negatively associated with 12 proteins (CLEC1B, CTSC, CTSS, gal-8, GCP5, NAAA, NrCAM, NTRK2, PLXNB3, RSPO1, sFRP3, and THY1). After adjustment for CRF, muscular strength was positively associated with eight proteins (BetaNGF, CDH6, CLEC10A, FcRL2, LAIR2, MSR1, SCARB2, and TNFRSF12A) and negatively associated with two proteins (gal-8 and NrCAM). After applying FDR correction, only CLEC10A remained statistically significant. In conclusion, muscular strength was associated with blood circulating proteins involved in several biological processes, particularly anti-inflammatory response, lipid metabolism, beta amyloid clearance, and neuronal action potential propagation. More powered studies are warranted in pediatric populations to contrast or confirm our findings.",

author = "Marcos Olvera-Rojas and Abel Plaza-Florido and Patricio Solis-Urra and Mar{\'i}a Rodriguez-Ayllon and Angel Toval and Irene Esteban-Cornejo and Ortega, \{Francisco B.\}",

note = "Funding Information: The present study was supported by grants from the Spanish Ministry of Economy and Competitiveness (DEP2013‐47540, DEP2016‐79512‐R, and DEP2017‐91544‐EXP), European Regional Development Fund (ERDF) of the European Commission (Number 667302), and by the Alicia Koplowitz Foundation. Supplementary funding was obtained from the Andalusian Operational Programme supported with ERDF (FEDER in Spanish, B‐CTS‐355‐UGR18). This study was also supported by the University of Granada, Plan Propio de Investigaci{\'o}n, Visiting Scholar grants and Excellence actions: Units of Excellence; Unit of Excellence on Exercise, Nutrition and Health (UCEENS) and by the Junta de Andaluc{\'i}a, Consejer{\'i}a de Conocimiento, Investigaci{\'o}n y Universidades and the ERDF (SOMM17/6107/UGR). The present study was further supported by the EXERNET Research Network on Exercise and Health (DEP2005‐00046/ACTI) and by the High Council of Sports (09/UPB/19). AP‐F contribution was funded in part by NIH grant \#: U01TR002004 (project REACH). MR‐A was supported by the Alicia Koplowitz Foundation. IE‐C is supported by the Spanish Ministry of Science and Innovation (RyC2019‐027287‐1). PS‐U is supported by a grant from ANID/BECAS Chile/72180543 and through a Margarita Salas grant from the Spanish Ministry Universities. AT is supported by the Junta de Andaluc{\'i}a postdoctoral research grant. Funding for open access charge: Universidad de Granada / CBUA. This work is part of Ph.D. Thesis conducted in the Biomedicine Doctoral Studies of the University of Granada, Spain. Publisher Copyright: {\textcopyright} 2023 The Authors. Scandinavian Journal of Medicine \& Science In Sports published by John Wiley \& Sons Ltd.",

year = "2023",

month = sep,

doi = "10.1111/sms.14387",

language = "English",

volume = "33",

pages = "1738--1751",

journal = "Scandinavian Journal of Medicine and Science in Sports",

issn = "0905-7188",

publisher = "Blackwell Publishing",

number = "9",

}

Association of muscular strength and targeted proteomics involved in brain health in children with overweight/obesity. / Olvera-Rojas, Marcos; Plaza-Florido, Abel; Solis-Urra, Patricio et al.
In: Scandinavian Journal of Medicine and Science in Sports, Vol. 33, No. 9, 09.2023, p. 1738-1751.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - Association of muscular strength and targeted proteomics involved in brain health in children with overweight/obesity

AU - Olvera-Rojas, Marcos

AU - Plaza-Florido, Abel

AU - Solis-Urra, Patricio

AU - Rodriguez-Ayllon, María

AU - Toval, Angel

AU - Esteban-Cornejo, Irene

AU - Ortega, Francisco B.

N1 - Funding Information: The present study was supported by grants from the Spanish Ministry of Economy and Competitiveness (DEP2013‐47540, DEP2016‐79512‐R, and DEP2017‐91544‐EXP), European Regional Development Fund (ERDF) of the European Commission (Number 667302), and by the Alicia Koplowitz Foundation. Supplementary funding was obtained from the Andalusian Operational Programme supported with ERDF (FEDER in Spanish, B‐CTS‐355‐UGR18). This study was also supported by the University of Granada, Plan Propio de Investigación, Visiting Scholar grants and Excellence actions: Units of Excellence; Unit of Excellence on Exercise, Nutrition and Health (UCEENS) and by the Junta de Andalucía, Consejería de Conocimiento, Investigación y Universidades and the ERDF (SOMM17/6107/UGR). The present study was further supported by the EXERNET Research Network on Exercise and Health (DEP2005‐00046/ACTI) and by the High Council of Sports (09/UPB/19). AP‐F contribution was funded in part by NIH grant #: U01TR002004 (project REACH). MR‐A was supported by the Alicia Koplowitz Foundation. IE‐C is supported by the Spanish Ministry of Science and Innovation (RyC2019‐027287‐1). PS‐U is supported by a grant from ANID/BECAS Chile/72180543 and through a Margarita Salas grant from the Spanish Ministry Universities. AT is supported by the Junta de Andalucía postdoctoral research grant. Funding for open access charge: Universidad de Granada / CBUA. This work is part of Ph.D. Thesis conducted in the Biomedicine Doctoral Studies of the University of Granada, Spain. Publisher Copyright: © 2023 The Authors. Scandinavian Journal of Medicine & Science In Sports published by John Wiley & Sons Ltd.

PY - 2023/9

Y1 - 2023/9

N2 - Muscular strength has been positively associated with better brain health indicators during childhood obesity. However, the molecular mechanisms underlying the positive impact of muscular strength in brain health are poorly understood. We aimed to study the association of muscular strength with neurology-related circulating proteins in plasma in children with overweight/obesity and to explore the role of cardiorespiratory fitness (CRF) as a confounder. The participants were 86 Caucasian children (10.1 ± 1.1 years old; 41% girls) from the ActiveBrains project. Muscular strength was measured by field and laboratory tests. CRF was assessed with an incremental treadmill test. Olink's technology was used to quantify 92 neurology-related proteins in plasma. Protein–protein interactions were computed using the STRING website. Muscular strength was positively associated with 12 proteins (BetaNGF, CDH6, CLEC10A, CLM1, FcRL2, HAGH, IL12, LAIR2, MSR1, SCARB2, SMOC2, and TNFRSF12A), and negatively associated with 12 proteins (CLEC1B, CTSC, CTSS, gal-8, GCP5, NAAA, NrCAM, NTRK2, PLXNB3, RSPO1, sFRP3, and THY1). After adjustment for CRF, muscular strength was positively associated with eight proteins (BetaNGF, CDH6, CLEC10A, FcRL2, LAIR2, MSR1, SCARB2, and TNFRSF12A) and negatively associated with two proteins (gal-8 and NrCAM). After applying FDR correction, only CLEC10A remained statistically significant. In conclusion, muscular strength was associated with blood circulating proteins involved in several biological processes, particularly anti-inflammatory response, lipid metabolism, beta amyloid clearance, and neuronal action potential propagation. More powered studies are warranted in pediatric populations to contrast or confirm our findings.

AB - Muscular strength has been positively associated with better brain health indicators during childhood obesity. However, the molecular mechanisms underlying the positive impact of muscular strength in brain health are poorly understood. We aimed to study the association of muscular strength with neurology-related circulating proteins in plasma in children with overweight/obesity and to explore the role of cardiorespiratory fitness (CRF) as a confounder. The participants were 86 Caucasian children (10.1 ± 1.1 years old; 41% girls) from the ActiveBrains project. Muscular strength was measured by field and laboratory tests. CRF was assessed with an incremental treadmill test. Olink's technology was used to quantify 92 neurology-related proteins in plasma. Protein–protein interactions were computed using the STRING website. Muscular strength was positively associated with 12 proteins (BetaNGF, CDH6, CLEC10A, CLM1, FcRL2, HAGH, IL12, LAIR2, MSR1, SCARB2, SMOC2, and TNFRSF12A), and negatively associated with 12 proteins (CLEC1B, CTSC, CTSS, gal-8, GCP5, NAAA, NrCAM, NTRK2, PLXNB3, RSPO1, sFRP3, and THY1). After adjustment for CRF, muscular strength was positively associated with eight proteins (BetaNGF, CDH6, CLEC10A, FcRL2, LAIR2, MSR1, SCARB2, and TNFRSF12A) and negatively associated with two proteins (gal-8 and NrCAM). After applying FDR correction, only CLEC10A remained statistically significant. In conclusion, muscular strength was associated with blood circulating proteins involved in several biological processes, particularly anti-inflammatory response, lipid metabolism, beta amyloid clearance, and neuronal action potential propagation. More powered studies are warranted in pediatric populations to contrast or confirm our findings.

UR - https://www.scopus.com/pages/publications/85159327427

U2 - 10.1111/sms.14387

DO - 10.1111/sms.14387

M3 - Article

C2 - 37190796

AN - SCOPUS:85159327427

SN - 0905-7188

VL - 33

SP - 1738

EP - 1751

JO - Scandinavian Journal of Medicine and Science in Sports

JF - Scandinavian Journal of Medicine and Science in Sports

IS - 9

ER -

Association of muscular strength and targeted proteomics involved in brain health in children with overweight/obesity

Abstract

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