ATP synthesis from 2,3-diphosphoglycerate by cell-free extract of Methanobacterium thermoautotrophicum (strain ΔH)

Gert Jan W.M. van Alebeek; Corné Klaassen; Jan T. Keltjens; Chris van der Drift; Godfried D. Vogels

doi:10.1007/BF00245397

ATP synthesis from 2,3-diphosphoglycerate by cell-free extract of Methanobacterium thermoautotrophicum (strain ΔH)

Gert Jan W.M. van Alebeek^*, Corné Klaassen, Jan T. Keltjens, Chris van der Drift, Godfried D. Vogels

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

8 Citations (Scopus)

Abstract

Cell-free extracts of Methanobacterium thermoautotrophicum were found to catalyze ATP synthesis from an endogeneous substrate. Synthesis was stimulated under hydrogen atmosphere and inhibited by KCL (K_i=150 mM). Comparison of the properties of a number of cell constituents showed the endogeneous substrate to be 2,3-diphosphoglycerate. The compound is converted into 3-phosphoglycerate, and via 2-phosphoglycerate and phosphoenolpyruvate into pyruvate, at which the latter reaction is linked with ATP synthesis.

Original language	English
Pages (from-to)	491-496
Number of pages	6
Journal	Archives of Microbiology
Volume	156
Issue number	6
DOIs	https://doi.org/10.1007/BF00245397
Publication status	Published - Nov 1991
Externally published	Yes

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title = "ATP synthesis from 2,3-diphosphoglycerate by cell-free extract of Methanobacterium thermoautotrophicum (strain ΔH)",

abstract = "Cell-free extracts of Methanobacterium thermoautotrophicum were found to catalyze ATP synthesis from an endogeneous substrate. Synthesis was stimulated under hydrogen atmosphere and inhibited by KCL (Ki=150 mM). Comparison of the properties of a number of cell constituents showed the endogeneous substrate to be 2,3-diphosphoglycerate. The compound is converted into 3-phosphoglycerate, and via 2-phosphoglycerate and phosphoenolpyruvate into pyruvate, at which the latter reaction is linked with ATP synthesis.",

author = "{van Alebeek}, {Gert Jan W.M.} and Corn{\'e} Klaassen and Keltjens, {Jan T.} and {van der Drift}, Chris and Vogels, {Godfried D.}",

year = "1991",

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doi = "10.1007/BF00245397",

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T1 - ATP synthesis from 2,3-diphosphoglycerate by cell-free extract of Methanobacterium thermoautotrophicum (strain ΔH)

AU - van Alebeek, Gert Jan W.M.

AU - Klaassen, Corné

AU - Keltjens, Jan T.

AU - van der Drift, Chris

AU - Vogels, Godfried D.

PY - 1991/11

Y1 - 1991/11

N2 - Cell-free extracts of Methanobacterium thermoautotrophicum were found to catalyze ATP synthesis from an endogeneous substrate. Synthesis was stimulated under hydrogen atmosphere and inhibited by KCL (Ki=150 mM). Comparison of the properties of a number of cell constituents showed the endogeneous substrate to be 2,3-diphosphoglycerate. The compound is converted into 3-phosphoglycerate, and via 2-phosphoglycerate and phosphoenolpyruvate into pyruvate, at which the latter reaction is linked with ATP synthesis.

AB - Cell-free extracts of Methanobacterium thermoautotrophicum were found to catalyze ATP synthesis from an endogeneous substrate. Synthesis was stimulated under hydrogen atmosphere and inhibited by KCL (Ki=150 mM). Comparison of the properties of a number of cell constituents showed the endogeneous substrate to be 2,3-diphosphoglycerate. The compound is converted into 3-phosphoglycerate, and via 2-phosphoglycerate and phosphoenolpyruvate into pyruvate, at which the latter reaction is linked with ATP synthesis.

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U2 - 10.1007/BF00245397

DO - 10.1007/BF00245397

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AN - SCOPUS:0026009315

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SP - 491

EP - 496

JO - Archives of Microbiology

JF - Archives of Microbiology

IS - 6

ER -

ATP synthesis from 2,3-diphosphoglycerate by cell-free extract of Methanobacterium thermoautotrophicum (strain ΔH)

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