ATP synthesis from 2,3-diphosphoglycerate by cell-free extract of Methanobacterium thermoautotrophicum (strain ΔH)

Gert Jan W.M. van Alebeek; Corné Klaassen; Jan T. Keltjens; Chris van der Drift; Godfried D. Vogels

doi:10.1007/BF00245397

ATP synthesis from 2,3-diphosphoglycerate by cell-free extract of Methanobacterium thermoautotrophicum (strain ΔH)

Gert Jan W.M. van Alebeek^*
, Corné Klaassen
, Jan T. Keltjens
, Chris van der Drift
, Godfried D. Vogels

^*Corresponding author for this work

Radboud University Nijmegen
Radboud University Medical Center

Research output: Contribution to journal › Article › Academic › peer-review

9 Citations (Scopus)

Abstract

Cell-free extracts of Methanobacterium thermoautotrophicum were found to catalyze ATP synthesis from an endogeneous substrate. Synthesis was stimulated under hydrogen atmosphere and inhibited by KCL (K_i=150 mM). Comparison of the properties of a number of cell constituents showed the endogeneous substrate to be 2,3-diphosphoglycerate. The compound is converted into 3-phosphoglycerate, and via 2-phosphoglycerate and phosphoenolpyruvate into pyruvate, at which the latter reaction is linked with ATP synthesis.

Original language	English
Pages (from-to)	491-496
Number of pages	6
Journal	Archives of Microbiology
Volume	156
Issue number	6
DOIs	https://doi.org/10.1007/BF00245397
Publication status	Published - Nov 1991
Externally published	Yes

Access to Document

10.1007/BF00245397

Cite this

@article{2cf096193f654d539aa9e085088434c1,

title = "ATP synthesis from 2,3-diphosphoglycerate by cell-free extract of Methanobacterium thermoautotrophicum (strain ΔH)",

abstract = "Cell-free extracts of Methanobacterium thermoautotrophicum were found to catalyze ATP synthesis from an endogeneous substrate. Synthesis was stimulated under hydrogen atmosphere and inhibited by KCL (Ki=150 mM). Comparison of the properties of a number of cell constituents showed the endogeneous substrate to be 2,3-diphosphoglycerate. The compound is converted into 3-phosphoglycerate, and via 2-phosphoglycerate and phosphoenolpyruvate into pyruvate, at which the latter reaction is linked with ATP synthesis.",

author = "\{van Alebeek\}, \{Gert Jan W.M.\} and Corn{\'e} Klaassen and Keltjens, \{Jan T.\} and \{van der Drift\}, Chris and Vogels, \{Godfried D.\}",

year = "1991",

month = nov,

doi = "10.1007/BF00245397",

language = "English",

volume = "156",

pages = "491--496",

journal = "Archives of Microbiology",

issn = "0302-8933",

publisher = "Springer Science+Business Media",

number = "6",

}

TY - JOUR

T1 - ATP synthesis from 2,3-diphosphoglycerate by cell-free extract of Methanobacterium thermoautotrophicum (strain ΔH)

AU - van Alebeek, Gert Jan W.M.

AU - Klaassen, Corné

AU - Keltjens, Jan T.

AU - van der Drift, Chris

AU - Vogels, Godfried D.

PY - 1991/11

Y1 - 1991/11

N2 - Cell-free extracts of Methanobacterium thermoautotrophicum were found to catalyze ATP synthesis from an endogeneous substrate. Synthesis was stimulated under hydrogen atmosphere and inhibited by KCL (Ki=150 mM). Comparison of the properties of a number of cell constituents showed the endogeneous substrate to be 2,3-diphosphoglycerate. The compound is converted into 3-phosphoglycerate, and via 2-phosphoglycerate and phosphoenolpyruvate into pyruvate, at which the latter reaction is linked with ATP synthesis.

AB - Cell-free extracts of Methanobacterium thermoautotrophicum were found to catalyze ATP synthesis from an endogeneous substrate. Synthesis was stimulated under hydrogen atmosphere and inhibited by KCL (Ki=150 mM). Comparison of the properties of a number of cell constituents showed the endogeneous substrate to be 2,3-diphosphoglycerate. The compound is converted into 3-phosphoglycerate, and via 2-phosphoglycerate and phosphoenolpyruvate into pyruvate, at which the latter reaction is linked with ATP synthesis.

UR - https://www.scopus.com/pages/publications/0026009315

U2 - 10.1007/BF00245397

DO - 10.1007/BF00245397

M3 - Article

AN - SCOPUS:0026009315

SN - 0302-8933

VL - 156

SP - 491

EP - 496

JO - Archives of Microbiology

JF - Archives of Microbiology

IS - 6

ER -

ATP synthesis from 2,3-diphosphoglycerate by cell-free extract of Methanobacterium thermoautotrophicum (strain ΔH)

Abstract

Access to Document

Other files and links

Fingerprint

Cite this