B and T cell imbalances in CSF of patients with Hu-antibody associated PNS

Miranda de Graaf; J Beukelaar; J (Jelmer) Bergsma; Jaco Kraan; Martin van den Bent; Markus Klimek; Yvette van Norden; A Kusuma; Peter Sillevis Smitt; Jan willem Gratama

doi:10.1016/j.jneuroim.2008.01.007

B and T cell imbalances in CSF of patients with Hu-antibody associated PNS

Miranda de Graaf, J Beukelaar, J (Jelmer) Bergsma, Jaco Kraan, Martin van den Bent, Markus Klimek, Yvette van Norden, A Kusuma, Peter Sillevis Smitt, Jan willem Gratama

Research output: Contribution to journal › Article › Academic › peer-review

26 Citations (Scopus)

Abstract

In paraneoplastic neurological syndromes associated with Hu-antibodies (Hu-PNS) an important role for cellular immunity is hypothesized. We characterized the cerebrospinal fluid (CSF) pleocytosis in Hu-PNS patients by assessing the major lymphocyte subsets by flow cytometry. The B cell subset in the CSF of Hu-PNS patients showed a significant absolute (similar to 20x) and relative (similar to 3x) expansion, while the numbers of CD4+ T cells, CD8+ T cells and NK cells only showed an absolute expansion (similar to 4-7x) compared to the controls. On the other hand, the NKT cell subset showed a significant relative reduction in CSF and in blood of Hu-PNS patients. The relative B cell expansion is consistent with the intrathecal synthesis of Hu-antibodies, while the increased number of T and NK cells supports an additional role for cellular immunity in the pathogenesis of Hu-PNS. In addition, the autoimmune hypothesis of Hu-PNS is supported by the relative NKT cell deficiency. (C) 2008 Elsevier B.V. All rights reserved.

Original language	Undefined/Unknown
Pages (from-to)	164-170
Number of pages	7
Journal	Journal of Neuroimmunology
Volume	195
Issue number	1-2
DOIs	https://doi.org/10.1016/j.jneuroim.2008.01.007
Publication status	Published - 2008

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EMC MM-03-44-06
EMC MM-03-86-12

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10.1016/j.jneuroim.2008.01.007

http://hdl.handle.net/1765/29282

Cite this

@article{b89a56dc35e74123949697ff6fe6e57b,

title = "B and T cell imbalances in CSF of patients with Hu-antibody associated PNS",

abstract = "In paraneoplastic neurological syndromes associated with Hu-antibodies (Hu-PNS) an important role for cellular immunity is hypothesized. We characterized the cerebrospinal fluid (CSF) pleocytosis in Hu-PNS patients by assessing the major lymphocyte subsets by flow cytometry. The B cell subset in the CSF of Hu-PNS patients showed a significant absolute (similar to 20x) and relative (similar to 3x) expansion, while the numbers of CD4+ T cells, CD8+ T cells and NK cells only showed an absolute expansion (similar to 4-7x) compared to the controls. On the other hand, the NKT cell subset showed a significant relative reduction in CSF and in blood of Hu-PNS patients. The relative B cell expansion is consistent with the intrathecal synthesis of Hu-antibodies, while the increased number of T and NK cells supports an additional role for cellular immunity in the pathogenesis of Hu-PNS. In addition, the autoimmune hypothesis of Hu-PNS is supported by the relative NKT cell deficiency. (C) 2008 Elsevier B.V. All rights reserved.",

author = "{de Graaf}, Miranda and J Beukelaar and Bergsma, {J (Jelmer)} and Jaco Kraan and {van den Bent}, Martin and Markus Klimek and {van Norden}, Yvette and A Kusuma and {Sillevis Smitt}, Peter and Gratama, {Jan willem}",

year = "2008",

doi = "10.1016/j.jneuroim.2008.01.007",

language = "Undefined/Unknown",

volume = "195",

pages = "164--170",

journal = "Journal of Neuroimmunology",

issn = "0165-5728",

publisher = "Elsevier",

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TY - JOUR

T1 - B and T cell imbalances in CSF of patients with Hu-antibody associated PNS

AU - de Graaf, Miranda

AU - Beukelaar, J

AU - Bergsma, J (Jelmer)

AU - Kraan, Jaco

AU - van den Bent, Martin

AU - Klimek, Markus

AU - van Norden, Yvette

AU - Kusuma, A

AU - Sillevis Smitt, Peter

AU - Gratama, Jan willem

PY - 2008

Y1 - 2008

N2 - In paraneoplastic neurological syndromes associated with Hu-antibodies (Hu-PNS) an important role for cellular immunity is hypothesized. We characterized the cerebrospinal fluid (CSF) pleocytosis in Hu-PNS patients by assessing the major lymphocyte subsets by flow cytometry. The B cell subset in the CSF of Hu-PNS patients showed a significant absolute (similar to 20x) and relative (similar to 3x) expansion, while the numbers of CD4+ T cells, CD8+ T cells and NK cells only showed an absolute expansion (similar to 4-7x) compared to the controls. On the other hand, the NKT cell subset showed a significant relative reduction in CSF and in blood of Hu-PNS patients. The relative B cell expansion is consistent with the intrathecal synthesis of Hu-antibodies, while the increased number of T and NK cells supports an additional role for cellular immunity in the pathogenesis of Hu-PNS. In addition, the autoimmune hypothesis of Hu-PNS is supported by the relative NKT cell deficiency. (C) 2008 Elsevier B.V. All rights reserved.

AB - In paraneoplastic neurological syndromes associated with Hu-antibodies (Hu-PNS) an important role for cellular immunity is hypothesized. We characterized the cerebrospinal fluid (CSF) pleocytosis in Hu-PNS patients by assessing the major lymphocyte subsets by flow cytometry. The B cell subset in the CSF of Hu-PNS patients showed a significant absolute (similar to 20x) and relative (similar to 3x) expansion, while the numbers of CD4+ T cells, CD8+ T cells and NK cells only showed an absolute expansion (similar to 4-7x) compared to the controls. On the other hand, the NKT cell subset showed a significant relative reduction in CSF and in blood of Hu-PNS patients. The relative B cell expansion is consistent with the intrathecal synthesis of Hu-antibodies, while the increased number of T and NK cells supports an additional role for cellular immunity in the pathogenesis of Hu-PNS. In addition, the autoimmune hypothesis of Hu-PNS is supported by the relative NKT cell deficiency. (C) 2008 Elsevier B.V. All rights reserved.

U2 - 10.1016/j.jneuroim.2008.01.007

DO - 10.1016/j.jneuroim.2008.01.007

M3 - Article

C2 - 18358542

SN - 0165-5728

VL - 195

SP - 164

EP - 170

JO - Journal of Neuroimmunology

JF - Journal of Neuroimmunology

IS - 1-2

ER -