Bergmann Glial AMPA Receptors Are Required for Fine Motor Coordination

AS Saab; A Neumeyer; HM Jahn; Alexander Cupido; AAM Simek; Henk-jan Boele; A Scheller; K Le Meur; M Gotz; H Monyer; R Sprengel; ME Rubio; JW Deitmer; Chris de Zeeuw; F Kirchhoff

doi:10.1126/science.1221140

Bergmann Glial AMPA Receptors Are Required for Fine Motor Coordination

AS Saab, A Neumeyer, HM Jahn, Alexander Cupido, AAM Simek, Henk-jan Boele, A Scheller, K Le Meur, M Gotz, H Monyer, R Sprengel, ME Rubio, JW Deitmer, Chris de Zeeuw, F Kirchhoff

Neurosciences

Research output: Contribution to journal › Article › Academic › peer-review

145 Citations (Scopus)

Abstract

The impact of glial neurotransmitter receptors in vivo is still elusive. In the cerebellum, Bergmann glial (BG) cells express alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)-type glutamate receptors (AMPARs) composed exclusively of GluA1 and/or GluA4 subunits. With the use of conditional gene inactivation, we found that the majority of cerebellar GluA1/A4-type AMPARs are expressed in BG cells. In young mice, deletion of BG AMPARs resulted in retraction of glial appendages from Purkinje cell (PC) synapses, increased amplitude and duration of evoked PC currents, and a delayed formation of glutamatergic synapses. In adult mice, AMPAR inactivation also caused retraction of glial processes. The physiological and structural changes were accompanied by behavioral impairments in fine motor coordination. Thus, BG AMPARs are essential to optimize synaptic integration and cerebellar output function throughout life.

Original language	Undefined/Unknown
Pages (from-to)	749-753
Number of pages	5
Journal	Science
Volume	337
Issue number	6095
DOIs	https://doi.org/10.1126/science.1221140
Publication status	Published - 2012

Access to Document

10.1126/science.1221140

http://hdl.handle.net/1765/71740

Cite this

@article{1831639892524cd79a73de7fa54c80df,

title = "Bergmann Glial AMPA Receptors Are Required for Fine Motor Coordination",

abstract = "The impact of glial neurotransmitter receptors in vivo is still elusive. In the cerebellum, Bergmann glial (BG) cells express alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)-type glutamate receptors (AMPARs) composed exclusively of GluA1 and/or GluA4 subunits. With the use of conditional gene inactivation, we found that the majority of cerebellar GluA1/A4-type AMPARs are expressed in BG cells. In young mice, deletion of BG AMPARs resulted in retraction of glial appendages from Purkinje cell (PC) synapses, increased amplitude and duration of evoked PC currents, and a delayed formation of glutamatergic synapses. In adult mice, AMPAR inactivation also caused retraction of glial processes. The physiological and structural changes were accompanied by behavioral impairments in fine motor coordination. Thus, BG AMPARs are essential to optimize synaptic integration and cerebellar output function throughout life.",

author = "AS Saab and A Neumeyer and HM Jahn and Alexander Cupido and AAM Simek and Henk-jan Boele and A Scheller and {Le Meur}, K and M Gotz and H Monyer and R Sprengel and ME Rubio and JW Deitmer and {de Zeeuw}, Chris and F Kirchhoff",

year = "2012",

doi = "10.1126/science.1221140",

language = "Undefined/Unknown",

volume = "337",

pages = "749--753",

journal = "Science",

issn = "0036-8075",

publisher = "American Association for the Advancement of Science",

number = "6095",

}

TY - JOUR

T1 - Bergmann Glial AMPA Receptors Are Required for Fine Motor Coordination

AU - Saab, AS

AU - Neumeyer, A

AU - Jahn, HM

AU - Cupido, Alexander

AU - Simek, AAM

AU - Boele, Henk-jan

AU - Scheller, A

AU - Le Meur, K

AU - Gotz, M

AU - Monyer, H

AU - Sprengel, R

AU - Rubio, ME

AU - Deitmer, JW

AU - de Zeeuw, Chris

AU - Kirchhoff, F

PY - 2012

Y1 - 2012

N2 - The impact of glial neurotransmitter receptors in vivo is still elusive. In the cerebellum, Bergmann glial (BG) cells express alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)-type glutamate receptors (AMPARs) composed exclusively of GluA1 and/or GluA4 subunits. With the use of conditional gene inactivation, we found that the majority of cerebellar GluA1/A4-type AMPARs are expressed in BG cells. In young mice, deletion of BG AMPARs resulted in retraction of glial appendages from Purkinje cell (PC) synapses, increased amplitude and duration of evoked PC currents, and a delayed formation of glutamatergic synapses. In adult mice, AMPAR inactivation also caused retraction of glial processes. The physiological and structural changes were accompanied by behavioral impairments in fine motor coordination. Thus, BG AMPARs are essential to optimize synaptic integration and cerebellar output function throughout life.

AB - The impact of glial neurotransmitter receptors in vivo is still elusive. In the cerebellum, Bergmann glial (BG) cells express alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)-type glutamate receptors (AMPARs) composed exclusively of GluA1 and/or GluA4 subunits. With the use of conditional gene inactivation, we found that the majority of cerebellar GluA1/A4-type AMPARs are expressed in BG cells. In young mice, deletion of BG AMPARs resulted in retraction of glial appendages from Purkinje cell (PC) synapses, increased amplitude and duration of evoked PC currents, and a delayed formation of glutamatergic synapses. In adult mice, AMPAR inactivation also caused retraction of glial processes. The physiological and structural changes were accompanied by behavioral impairments in fine motor coordination. Thus, BG AMPARs are essential to optimize synaptic integration and cerebellar output function throughout life.

U2 - 10.1126/science.1221140

DO - 10.1126/science.1221140

M3 - Article

SN - 0036-8075

VL - 337

SP - 749

EP - 753

JO - Science

JF - Science

IS - 6095

ER -