Bio-inspired polymeric iron-doped hydroxyapatite microspheres as a tunable carrier of rhBMP-2

TMF Patricio, Didem Mumcuoglu, M Montesi, S Panseri, Janneke Witte - Bouma, Shorouk Fahmy Garcia, M Sandri, A Tampieri, Eric Farrell, S Sprio

Research output: Contribution to journalArticleAcademicpeer-review

4 Citations (Scopus)

Abstract

Hybrid superparamagnetic microspheres with bone-like composition, previously developed by a bio-inspired assembling/mineralization process, are evaluated for their ability to uptake and deliver recombinant human bone morphogenetic protein-2 (rhBMP-2) in therapeutically-relevant doses along with prolonged release profiles. The comparison with hybrid non-magnetic and with non-mineralized microspheres highlights the role of nanocrystalline, nanosize mineral phases when they exhibit surface charged groups enabling the chemical linking with the growth factor and thus moderating the release kinetics. All the microspheres show excellent osteogenic ability with human mesenchymal stem cells whereas the hybrid mineralized ones show a slow and sustained release of rhBMP-2 along 14 days of soaking into cell culture medium with substantially bioactive effect, as reported by assay with C2C12 BRE-Luc cell line. It is also shown that the release extent can be modulated by the application of pulsed electromagnetic field, thus showing the potential of remote controlling the bioactivity of the new micro-devices which is promising for future application of hybrid biomimetic microspheres in precisely designed and personalized therapies.

Original languageEnglish
Article number111410
JournalMaterials Science & Engineering C-Biomimetic Materials Sensors and Systems
Volume119
DOIs
Publication statusPublished - Feb 2021

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