The incidence of overweight and obesity in men of reproductive ages is rising, which may affect fertility. Therefore, this study aims to assess the associations between BMI, central adiposity and sperm parameters in men of subfertile couples. Ejaculate volume (ml), sperm concentration (millions per ml), percentage of progressive motile and immotile spermatozoa and total motile sperm count (millions) were measured in 450 men of subfertile couples visiting a tertiary outpatient clinic for reproductive treatment and preconception counseling. Overweight was negatively associated with the percentage of progressive motility type A [ 0.32 (SE 0.2), P 0.036] and positively associated with the percentage of immotility type C [ 0.21 (SE 0.07), P 0.002]. Obesity was negatively associated with ejaculate volume [ 0.23 (SE 0.1), P 0.02], sperm concentration [ 0.77 (SE 0.3), P 0.006] and total motile sperm count [ 0.91 (SE 0.3), P 0.007]. Waist circumference epsilon 102 cm, a measure for central adiposity, was inversely associated with sperm co This study shows that in particular, sperm concentration and total motile sperm count in men of subfertile couples are detrimentally affected by a high BMI and central adiposity. The effect of weight loss on sperm quality and fertility needs further investigation.