BRCA2 binding through a cryptic repeated motif to HSF2BP oligomers does not impact meiotic recombination

Rania Ghouil, Simona Miron, Lieke Koornneef, Jasper Veerman, Maarten W. Paul, Marie Hélène Le Du, Esther Sleddens-Linkels, Sari E. van Rossum-Fikkert, Yvette van Loon, Natalia Felipe-Medina, Alberto M. Pendas, Alex Maas, Jeroen Essers, Pierre Legrand, Willy M. Baarends, Roland Kanaar*, Sophie Zinn-Justin*, Alex N. Zelensky*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

5 Citations (Scopus)

Abstract

BRCA2 and its interactors are required for meiotic homologous recombination (HR) and fertility. Loss of HSF2BP, a BRCA2 interactor, disrupts HR during spermatogenesis. We test the model postulating that HSF2BP localizes BRCA2 to meiotic HR sites, by solving the crystal structure of the BRCA2 fragment in complex with dimeric armadillo domain (ARM) of HSF2BP and disrupting this interaction in a mouse model. This reveals a repeated 23 amino acid motif in BRCA2, each binding the same conserved surface of one ARM domain. In the complex, two BRCA2 fragments hold together two ARM dimers, through a large interface responsible for the nanomolar affinity — the strongest interaction involving BRCA2 measured so far. Deleting exon 12, encoding the first repeat, from mBrca2 disrupts BRCA2 binding to HSF2BP, but does not phenocopy HSF2BP loss. Thus, results herein suggest that the high-affinity oligomerization-inducing BRCA2-HSF2BP interaction is not required for RAD51 and DMC1 recombinase localization in meiotic HR.

Original languageEnglish
Article number4605
JournalNature Communications
Volume12
Issue number1
DOIs
Publication statusPublished - 29 Jul 2021

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