Capture of circulatory endothelial progenitor cells and accelerated re-endothelialization of a bio-engineered stent in human ex vivo shunt and rabbit denudation model

Katarina Larsen, Caroline Cheng, Dennie Tempel, S Parker, S Yazdani, Wijnand den Dekker, Jaco Houtgraaf, Renate Jong, S Swager-ten Hoor, E Ligtenberg, SR Hanson, S Rowland, F Kolodgie, PWJC (Patrick) Serruys, R Virmani, Eric Duckers

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Abstract

Aims The Genous (TM) Bio-engineered R (TM) stent (GS) aims to promote vascular healing by capture of circulatory endothelial progenitor cells (EPCs) to the surface of the stent struts, resulting in accelerated re-endothelialization. Here, we assessed the function of the GS in comparison to bare-metal stent (BMS), when exposed to the human and animal circulation. Methods and results First, 15 patients undergoing coronary angiography received an extracorporeal femoral arteriovenous (AV) shunt containing BMS and GS. Macroscopical mural thrombi were observed in BMS, whereas GS remained visibly clean. Confocal and scanning electron microscopic (SEM) analysis of GS showed an increase in strut coverage. Quantitative polymerase chain reaction (qPCR) analysis of captured cells on the GS demonstrated increased expression of endothelial markers KDR/VEGFR2 and E-se Conclusion In this proof-of-concept study, we have demonstrated that the bio-engineered EPC-capture stent, Genous (TM) R (TM) stent, is effective in EPC capture, resulting in accelerated re-endothelialization and reduced thrombogenicity.
Original languageUndefined/Unknown
Pages (from-to)120-128
Number of pages9
JournalEuropean Heart Journal
Volume33
Issue number1
DOIs
Publication statusPublished - 2012

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