CD40L stimulation of rat dendritic cells specifically favors the IL-12/IL-10 ratio resulting in a strong T cell stimulatory capacity

Annelein M. Stax; Cynthia Crul; Sylvia W.A. Kamerling; Nicole Schlagwein; Reinier N. van der Geest; Andrea M. Woltman; Cees van Kooten

doi:10.1016/j.molimm.2007.12.014

CD40L stimulation of rat dendritic cells specifically favors the IL-12/IL-10 ratio resulting in a strong T cell stimulatory capacity

Annelein M. Stax, Cynthia Crul, Sylvia W.A. Kamerling, Nicole Schlagwein, Reinier N. van der Geest, Andrea M. Woltman, Cees van Kooten^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

12 Citations (Scopus)

Abstract

Dendritic cells (DC) play an important role in immune responses and have been studied extensively in human and mouse models. CD40 triggering of DC has a pivotal role in their maturation process, obtaining the unique capacity to induce strong CD4 and CD8 T cell activation. Although rat models are frequently used for the understanding of the underlying mechanism of human diseases, relatively little is known about rat DC. To investigate the effect of CD40 triggering on rat DC, we cloned the rat CD40L gene and generated murine fibroblasts with stable expression (L-rCD40L). DC stimulated by L-rCD40L cells exhibited a strong T cell stimulatory capacity, associated with higher amounts of IFN-γ as compared to LPS-stimulated DC. Analysis of cytokine production showed that LPS induced both IL-12 and IL-10 production, whereas CD40L induced high amounts of IL-12, but little IL-10 production by rat DC. This implies that the difference found in T cell stimulatory capacity by the stimulated DC is due to the cytokine profile of the DC at the time of T cell activation.

Original language	English
Pages (from-to)	2641-2650
Number of pages	10
Journal	Molecular Immunology
Volume	45
Issue number	9
Early online date	8 Feb 2008
DOIs	https://doi.org/10.1016/j.molimm.2007.12.014
Publication status	Published - May 2008
Externally published	Yes

Bibliographical note

Funding Information:
This work was supported by the EU grants QLRT-2001-01215 and LSHB-CT-2004-512090 (RISET) and the JDRF/DF/Zon-MW centergrant #2003.10.002

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.molimm.2007.12.014

Cite this

@article{d3bf6cb6ca0942b28783450530ae1b68,

title = "CD40L stimulation of rat dendritic cells specifically favors the IL-12/IL-10 ratio resulting in a strong T cell stimulatory capacity",

abstract = "Dendritic cells (DC) play an important role in immune responses and have been studied extensively in human and mouse models. CD40 triggering of DC has a pivotal role in their maturation process, obtaining the unique capacity to induce strong CD4 and CD8 T cell activation. Although rat models are frequently used for the understanding of the underlying mechanism of human diseases, relatively little is known about rat DC. To investigate the effect of CD40 triggering on rat DC, we cloned the rat CD40L gene and generated murine fibroblasts with stable expression (L-rCD40L). DC stimulated by L-rCD40L cells exhibited a strong T cell stimulatory capacity, associated with higher amounts of IFN-γ as compared to LPS-stimulated DC. Analysis of cytokine production showed that LPS induced both IL-12 and IL-10 production, whereas CD40L induced high amounts of IL-12, but little IL-10 production by rat DC. This implies that the difference found in T cell stimulatory capacity by the stimulated DC is due to the cytokine profile of the DC at the time of T cell activation.",

author = "Stax, {Annelein M.} and Cynthia Crul and Kamerling, {Sylvia W.A.} and Nicole Schlagwein and {van der Geest}, {Reinier N.} and Woltman, {Andrea M.} and {van Kooten}, Cees",

note = "Funding Information: This work was supported by the EU grants QLRT-2001-01215 and LSHB-CT-2004-512090 (RISET) and the JDRF/DF/Zon-MW centergrant #2003.10.002",

year = "2008",

month = may,

doi = "10.1016/j.molimm.2007.12.014",

language = "English",

volume = "45",

pages = "2641--2650",

journal = "Molecular Immunology",

issn = "0161-5890",

publisher = "Elsevier Ltd.",

number = "9",

}

TY - JOUR

T1 - CD40L stimulation of rat dendritic cells specifically favors the IL-12/IL-10 ratio resulting in a strong T cell stimulatory capacity

AU - Stax, Annelein M.

AU - Crul, Cynthia

AU - Kamerling, Sylvia W.A.

AU - Schlagwein, Nicole

AU - van der Geest, Reinier N.

AU - Woltman, Andrea M.

AU - van Kooten, Cees

N1 - Funding Information: This work was supported by the EU grants QLRT-2001-01215 and LSHB-CT-2004-512090 (RISET) and the JDRF/DF/Zon-MW centergrant #2003.10.002

PY - 2008/5

Y1 - 2008/5

N2 - Dendritic cells (DC) play an important role in immune responses and have been studied extensively in human and mouse models. CD40 triggering of DC has a pivotal role in their maturation process, obtaining the unique capacity to induce strong CD4 and CD8 T cell activation. Although rat models are frequently used for the understanding of the underlying mechanism of human diseases, relatively little is known about rat DC. To investigate the effect of CD40 triggering on rat DC, we cloned the rat CD40L gene and generated murine fibroblasts with stable expression (L-rCD40L). DC stimulated by L-rCD40L cells exhibited a strong T cell stimulatory capacity, associated with higher amounts of IFN-γ as compared to LPS-stimulated DC. Analysis of cytokine production showed that LPS induced both IL-12 and IL-10 production, whereas CD40L induced high amounts of IL-12, but little IL-10 production by rat DC. This implies that the difference found in T cell stimulatory capacity by the stimulated DC is due to the cytokine profile of the DC at the time of T cell activation.

AB - Dendritic cells (DC) play an important role in immune responses and have been studied extensively in human and mouse models. CD40 triggering of DC has a pivotal role in their maturation process, obtaining the unique capacity to induce strong CD4 and CD8 T cell activation. Although rat models are frequently used for the understanding of the underlying mechanism of human diseases, relatively little is known about rat DC. To investigate the effect of CD40 triggering on rat DC, we cloned the rat CD40L gene and generated murine fibroblasts with stable expression (L-rCD40L). DC stimulated by L-rCD40L cells exhibited a strong T cell stimulatory capacity, associated with higher amounts of IFN-γ as compared to LPS-stimulated DC. Analysis of cytokine production showed that LPS induced both IL-12 and IL-10 production, whereas CD40L induced high amounts of IL-12, but little IL-10 production by rat DC. This implies that the difference found in T cell stimulatory capacity by the stimulated DC is due to the cytokine profile of the DC at the time of T cell activation.

UR - http://www.scopus.com/inward/record.url?scp=40849097440&partnerID=8YFLogxK

U2 - 10.1016/j.molimm.2007.12.014

DO - 10.1016/j.molimm.2007.12.014

M3 - Article

C2 - 18262271

AN - SCOPUS:40849097440

SN - 0161-5890

VL - 45

SP - 2641

EP - 2650

JO - Molecular Immunology

JF - Molecular Immunology

IS - 9

ER -

CD40L stimulation of rat dendritic cells specifically favors the IL-12/IL-10 ratio resulting in a strong T cell stimulatory capacity

Abstract

Bibliographical note

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this