cDNA cloning and mRNA expression of the six mouse insulin-like growth factor binding proteins

A. G.P. Schuller*, C. Groffen, J. W. van Neck, E. C. Zwarthoff, S. L.S. Drop

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

63 Citations (Scopus)


The insulin-like growth factor binding proteins (IGFBPs) comprise a family of six distinct proteins which modulate insulin-like growth factor action. We have isolated cDNAs encoding the six mouse IGFBPs (mIGFBPs). In addition, we studied the mRNA expression of the six mIGFBPs during development and in various adult tissues. Our results show that each of the six mIGFBPs is highly homologous to their human and rat counterparts, whereas only the N and C terminal ends are conserved between the six mIGFBPs. Northern blotting revealed that mIGFBP-2, -3, -4 and -5 genes are already expressed at gestational day 11.5, suggesting a role for these mIGFBPs in embryonal development. In liver, a peak of mIGFBP-1 mRNA expression was found around birth, suggesting a function for mIGFBP-1 in the newborn mouse. Finally, tissue-specific expression of the six mouse IGFBP genes was observed in adult tissues suggesting different roles or modes of actions in adult life.

Original languageEnglish
Pages (from-to)57-66
Number of pages10
JournalMolecular and Cellular Endocrinology
Issue number1
Publication statusPublished - Aug 1994

Bibliographical note

Funding Information:
The authors would like to thank F.L. van der Panne for photography. This work was supported by grants from the Sophia Foundation for Medical Research, The Netherlands and Novo-Nordisk Insulin Laboratories, Denmark.


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