Cell-cell interactions during the formation of primordial follicles in humans

Sylwia M. Czukiewska, Xueying Fan, Adriaan A. Mulder, Talia Van Der Helm, Sanne Hillenius, Lotte Van Der Meeren, Roberto Matorras, Cristina Eguizabal, Lei Lei, Roman I. Koning, Susana M. Chuva De Sousa Lopes*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

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Abstract

Gametogenesis is a complex and sex-specific multistep process during which the gonadal somatic niche plays an essential regulatory role. One of the most crucial steps during human female gametogenesis is the formation of primordial follicles, the functional unit of the ovary that constitutes the pool of follicles available at birth during the entire reproductive life. However, the relation between human fetal germ cells (hFGCs) and gonadal somatic cells during the formation of the primordial follicles remains largely unexplored. We have discovered that hFGCs can form multinucleated syncytia, some connected via interconnecting intercellular bridges, and that not all nuclei in hFGC-syncytia were synchronous regarding meiotic stage. As hFGCs progressed in development, pre-granulosa cells formed protrusions that seemed to progressively constrict individual hFGCs, perhaps contributing to separate them from the multinucleated syncytia. Our findings highlighted the cell-cell interaction and molecular dynamics between hFGCs and (pre)granulosa cells during the formation of primordial follicles in humans. Knowledge on how the pool of primordial follicle is formed is important to understand human infertility.

Original languageEnglish
Article numbere202301926
JournalLife Science Alliance
Volume6
Issue number11
DOIs
Publication statusPublished - Nov 2023

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Publisher Copyright: © 2023 Czukiewska et al.

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