Characterization and Evaluation of the Artemis Camera for Fluorescence-Guided Cancer Surgery

Kishan Tsang, M (Martijn) van der Giessen, MC Boonstra, TJA Snoeks, Stijn Keereweer, S (Sabrina) Oliveira, CJH van de Velde, BPF Lelieveldt, AL Vahrmeijer, Clemens Löwik, J (Jouke) Dijkstra

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Near-infrared (NIR) fluorescence imaging can provide the surgeon with real-time visualization of, e.g., tumor margins and lymph nodes. We describe and evaluate the Artemis, a novel, handheld NIR fluorescence camera. We evaluated minimal detectable cell numbers (FaDu-luc2, 7D12-IRDye 800CW), preclinical intraoperative detection of sentinel lymph nodes (SLN) using indocyanine green (ICG), and of orthotopic tongue tumors using 7D12-800CW. Results were compared with the Pearl imager. Clinically, three patients with liver metastases were imaged using ICG. Minimum detectable cell counts for Artemis and Pearl were 2 x 10(5) and 4 x 10(4) cells, respectively. In vivo, seven SLNs were detected in four mice with both cameras. Orthotopic OSC-19-luc2-cGFP tongue tumors were clearly identifiable, and a minimum FaDu-luc2 tumor size of 1 mm(3) could be identified. Six human malignant lesions were identified during three liver surgery procedures. Based on this study, the Artemis system has demonstrated its utility in fluorescence-guided cancer surgery.
Original languageUndefined/Unknown
Pages (from-to)413-423
Number of pages11
JournalMolecular Imaging and Biology
Issue number3
Publication statusPublished - 2015

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