TY - JOUR
T1 - Characterization of a cDNA clone encoding molluscan insulin-related peptide V of Lymnaea stagnalis
AU - Smit, August B.
AU - Thijsen, Steven F.T.
AU - Geraerts, Wijnand P.M.
AU - Meester, Irene
AU - van Heerikshuizen, Harm
AU - Joosse, Joos
PY - 1992
Y1 - 1992
N2 - A cDNA clone encoding molluscan insulin-related peptide V (MIP V) was isolated from a cDNA library of the central nervous system (CNS) of the freshwater snail, Lymnaea stagnalis, using a heterologous screening with a previously identified MIP II cDNA The MIP V cDNA encodes a preprohormone resembling the organization of preproinsulin, with a putative signal sequence, and an A and B chain, however, in this case connected by two distinct C peptides, Cα and Cβ, instead of one single C peptide. This phenomenon, which is shared by the MIP II precursor, represents a new development in the prohormone organization of peptides belonging to the insulin superfamily. The A and B chains of MIPs V, I and II, differ remarkably in primary structure; in contrast, the Cα peptide domains are almost identical. MIP V has only limited sequence similarity with insulins and related peptides. Both MIP V and I exhibit structural features, which make them a unique class of the insulin superfamily. The MIP I, II and V genes are expressed in a single type of neuron the growth controlling neuroendocrine light green cells of the Lymnaea CNS.
AB - A cDNA clone encoding molluscan insulin-related peptide V (MIP V) was isolated from a cDNA library of the central nervous system (CNS) of the freshwater snail, Lymnaea stagnalis, using a heterologous screening with a previously identified MIP II cDNA The MIP V cDNA encodes a preprohormone resembling the organization of preproinsulin, with a putative signal sequence, and an A and B chain, however, in this case connected by two distinct C peptides, Cα and Cβ, instead of one single C peptide. This phenomenon, which is shared by the MIP II precursor, represents a new development in the prohormone organization of peptides belonging to the insulin superfamily. The A and B chains of MIPs V, I and II, differ remarkably in primary structure; in contrast, the Cα peptide domains are almost identical. MIP V has only limited sequence similarity with insulins and related peptides. Both MIP V and I exhibit structural features, which make them a unique class of the insulin superfamily. The MIP I, II and V genes are expressed in a single type of neuron the growth controlling neuroendocrine light green cells of the Lymnaea CNS.
M3 - Article
SN - 0006-8993
VL - 14
SP - 7
EP - 12
JO - Molecular Brain Research
JF - Molecular Brain Research
ER -