Characterization of four novel cag repeat-containing cDNAs

Jin Xing Jiang; Ronald H. Lekanne deprez; Ellen C. Zwarthoff; Peter H.J. Riegman

doi:10.1006/geno.1995.0015

Characterization of four novel cag repeat-containing cDNAs

Jin Xing Jiang
, Ronald H. Lekanne deprez
, Ellen C. Zwarthoff^*
, Peter H.J. Riegman

^*Corresponding author for this work

Pathology

SSM Health Cardinal Glennon Children's Medical Center
Amsterdam UMC
Erasmus University Medical Centre

Research output: Contribution to journal › Article › Academic › peer-review

15 Citations (Scopus)

Abstract

Stretches of CAG nucleotides coding for the amino acid glutamine are an important feature of many transcription factors and genes that are involved in neurodegenerative disorders. In an attempt to isolate CAG repeat- containing cDNAs expressed in nervous tissue, we screened a human fetal brain cDNA library with a probe containing a CAG repeat. Five different clones were characterized and found to contain CAG repeats. Sequence data revealed that four of these cDNAs were derived from novel genes. These cDNAs were designated CAG6, CAG12, CAG24, and CAG40 and were found to correspond to transcripts of 5.0, 7.5, 4.4, and 15 kb, respectively. The genes encoding CAG6, CAG12, CAG24, and CAG40 were assigned to chromosomes 12, 16, X, and 12, respectively. For the 5th gene, CAG26/pRHpA, a localization on two different chromosomes was established: 16 and X. None of the repeats showed any length polymorphisms in human DNA.

Original language	English
Pages (from-to)	91-93
Number of pages	3
Journal	Genomics
Volume	30
Issue number	1
DOIs	https://doi.org/10.1006/geno.1995.0015
Publication status	Published - Nov 1995

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title = "Characterization of four novel cag repeat-containing cDNAs",

abstract = "Stretches of CAG nucleotides coding for the amino acid glutamine are an important feature of many transcription factors and genes that are involved in neurodegenerative disorders. In an attempt to isolate CAG repeat- containing cDNAs expressed in nervous tissue, we screened a human fetal brain cDNA library with a probe containing a CAG repeat. Five different clones were characterized and found to contain CAG repeats. Sequence data revealed that four of these cDNAs were derived from novel genes. These cDNAs were designated CAG6, CAG12, CAG24, and CAG40 and were found to correspond to transcripts of 5.0, 7.5, 4.4, and 15 kb, respectively. The genes encoding CAG6, CAG12, CAG24, and CAG40 were assigned to chromosomes 12, 16, X, and 12, respectively. For the 5th gene, CAG26/pRHpA, a localization on two different chromosomes was established: 16 and X. None of the repeats showed any length polymorphisms in human DNA.",

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year = "1995",

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AU - Jiang, Jin Xing

AU - Lekanne deprez, Ronald H.

AU - Zwarthoff, Ellen C.

AU - Riegman, Peter H.J.

PY - 1995/11

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N2 - Stretches of CAG nucleotides coding for the amino acid glutamine are an important feature of many transcription factors and genes that are involved in neurodegenerative disorders. In an attempt to isolate CAG repeat- containing cDNAs expressed in nervous tissue, we screened a human fetal brain cDNA library with a probe containing a CAG repeat. Five different clones were characterized and found to contain CAG repeats. Sequence data revealed that four of these cDNAs were derived from novel genes. These cDNAs were designated CAG6, CAG12, CAG24, and CAG40 and were found to correspond to transcripts of 5.0, 7.5, 4.4, and 15 kb, respectively. The genes encoding CAG6, CAG12, CAG24, and CAG40 were assigned to chromosomes 12, 16, X, and 12, respectively. For the 5th gene, CAG26/pRHpA, a localization on two different chromosomes was established: 16 and X. None of the repeats showed any length polymorphisms in human DNA.

AB - Stretches of CAG nucleotides coding for the amino acid glutamine are an important feature of many transcription factors and genes that are involved in neurodegenerative disorders. In an attempt to isolate CAG repeat- containing cDNAs expressed in nervous tissue, we screened a human fetal brain cDNA library with a probe containing a CAG repeat. Five different clones were characterized and found to contain CAG repeats. Sequence data revealed that four of these cDNAs were derived from novel genes. These cDNAs were designated CAG6, CAG12, CAG24, and CAG40 and were found to correspond to transcripts of 5.0, 7.5, 4.4, and 15 kb, respectively. The genes encoding CAG6, CAG12, CAG24, and CAG40 were assigned to chromosomes 12, 16, X, and 12, respectively. For the 5th gene, CAG26/pRHpA, a localization on two different chromosomes was established: 16 and X. None of the repeats showed any length polymorphisms in human DNA.

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DO - 10.1006/geno.1995.0015

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SN - 0888-7543

VL - 30

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EP - 93

JO - Genomics

JF - Genomics

IS - 1

ER -

Characterization of four novel cag repeat-containing cDNAs

Abstract

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