Characterization of recombinant influenza A virus as a vector for HIV-1 p17(Gag)

Anna Goede, Patrick Boers, Lennard Dekker, Ab Osterhaus, Rob Gruters, Guus Rimmelzwaan

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11 Citations (Scopus)

Abstract

We have generated a recombinant influenza A virus with the HIV-1 p17(Gag) (rFlu-p17) gene inserted into the influenza virus neuraminidase (NA) gene. Expression of HIV-1 p17 protein was detected by conventional Western blot analysis and also by liquid chromatography/tandem mass spectrometry (LC-MS/MS) analysis of rFlu-p17 infected cells. The latter method does not depend on protein-specific antibody preparations and proved to be a powerful tool to detect proteins of interest. Next, antigen presentation of p17 expressed after infection of antigen-presenting cells was determined. Cloned p17-specific CD8+ T-cells were co-cultured with rFlu-p17 infected B-cells and produced IFN-gamma upon stimulation. Furthermore, we showed that immunization with rFlu-p17 elicited a humoral immune response in mice. This study shows that replication-deficient rFlu-p17 is antigenic in vitro and immunogenic in vivo and warrants further development as a candidate vaccine vector. (C) 2009 Elsevier Ltd. All rights reserved.
Original languageUndefined/Unknown
Pages (from-to)5735-5739
Number of pages5
JournalVaccine
Volume27
Issue number42
DOIs
Publication statusPublished - 2009

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