Characterization of the Prostate-specific Antigen gene: A novel human kallikrein-like gene

P. H.J. Riegman; R. J. Vlietstra; J. A.G.M. van der Korput; J. C. Romijn; J. Trapman

doi:10.1016/0006-291X(89)92409-1

Characterization of the Prostate-specific Antigen gene: A novel human kallikrein-like gene

P. H.J. Riegman^*, R. J. Vlietstra, J. A.G.M. van der Korput, J. C. Romijn, J. Trapman

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

155 Citations (Scopus)

Abstract

Using Prostate-specific Antigen cDNA fragments as hybridization probes a clone containing the information for the gene encoding Prostate-specific Antigen was isolated from a human genomic DNA library. The complete gene (about 6 kb) was sequenced and shown to be composed of four introns and five exons. Two major transcription initiation sites were found. The sequence of the promoter region revealed the presence of various well known transcription regulatory elements including a TATA box. A high percentage of homology was found between the Prostate-specific Antigen gene and the hGK-1 gene (82%). This homology extended into the promoter region. Two previously described variant Prostate-specific Antigen cDNAs can now be explained by intron retention and alternative splicing of the primary transcript.

Original language	English
Pages (from-to)	95-102
Number of pages	8
Journal	Biochemical and Biophysical Research Communications
Volume	159
Issue number	1
DOIs	https://doi.org/10.1016/0006-291X(89)92409-1
Publication status	Published - 28 Feb 1989

Bibliographical note

Funding Information:
We are indebted to Mrs. Paula Delfos for photography. by a grant from the Netherlands Cancer Society (KWF).

Access to Document

10.1016/0006-291X(89)92409-1

Cite this

@article{3ea4db640fef4ba2b75cc905169925b8,

title = "Characterization of the Prostate-specific Antigen gene: A novel human kallikrein-like gene",

abstract = "Using Prostate-specific Antigen cDNA fragments as hybridization probes a clone containing the information for the gene encoding Prostate-specific Antigen was isolated from a human genomic DNA library. The complete gene (about 6 kb) was sequenced and shown to be composed of four introns and five exons. Two major transcription initiation sites were found. The sequence of the promoter region revealed the presence of various well known transcription regulatory elements including a TATA box. A high percentage of homology was found between the Prostate-specific Antigen gene and the hGK-1 gene (82%). This homology extended into the promoter region. Two previously described variant Prostate-specific Antigen cDNAs can now be explained by intron retention and alternative splicing of the primary transcript.",

author = "Riegman, {P. H.J.} and Vlietstra, {R. J.} and {van der Korput}, {J. A.G.M.} and Romijn, {J. C.} and J. Trapman",

note = "Funding Information: We are indebted to Mrs. Paula Delfos for photography. by a grant from the Netherlands Cancer Society (KWF).",

year = "1989",

month = feb,

day = "28",

doi = "10.1016/0006-291X(89)92409-1",

language = "English",

volume = "159",

pages = "95--102",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Elsevier",

number = "1",

}

TY - JOUR

T1 - Characterization of the Prostate-specific Antigen gene

T2 - A novel human kallikrein-like gene

AU - Riegman, P. H.J.

AU - Vlietstra, R. J.

AU - van der Korput, J. A.G.M.

AU - Romijn, J. C.

AU - Trapman, J.

N1 - Funding Information: We are indebted to Mrs. Paula Delfos for photography. by a grant from the Netherlands Cancer Society (KWF).

PY - 1989/2/28

Y1 - 1989/2/28

N2 - Using Prostate-specific Antigen cDNA fragments as hybridization probes a clone containing the information for the gene encoding Prostate-specific Antigen was isolated from a human genomic DNA library. The complete gene (about 6 kb) was sequenced and shown to be composed of four introns and five exons. Two major transcription initiation sites were found. The sequence of the promoter region revealed the presence of various well known transcription regulatory elements including a TATA box. A high percentage of homology was found between the Prostate-specific Antigen gene and the hGK-1 gene (82%). This homology extended into the promoter region. Two previously described variant Prostate-specific Antigen cDNAs can now be explained by intron retention and alternative splicing of the primary transcript.

AB - Using Prostate-specific Antigen cDNA fragments as hybridization probes a clone containing the information for the gene encoding Prostate-specific Antigen was isolated from a human genomic DNA library. The complete gene (about 6 kb) was sequenced and shown to be composed of four introns and five exons. Two major transcription initiation sites were found. The sequence of the promoter region revealed the presence of various well known transcription regulatory elements including a TATA box. A high percentage of homology was found between the Prostate-specific Antigen gene and the hGK-1 gene (82%). This homology extended into the promoter region. Two previously described variant Prostate-specific Antigen cDNAs can now be explained by intron retention and alternative splicing of the primary transcript.

UR - http://www.scopus.com/inward/record.url?scp=0024596907&partnerID=8YFLogxK

U2 - 10.1016/0006-291X(89)92409-1

DO - 10.1016/0006-291X(89)92409-1

M3 - Article

C2 - 2466464

AN - SCOPUS:0024596907

SN - 0006-291X

VL - 159

SP - 95

EP - 102

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 1

ER -

Characterization of the Prostate-specific Antigen gene: A novel human kallikrein-like gene

Abstract

Bibliographical note

Access to Document

Other files and links

Fingerprint

Cite this