Childhood Adiposity Associated with Expanded Effector Memory CD8+and Vδ2+Vγ9+T Cells

Kirsten I.M. Looman, Susana Santos, Henriette A. Moll, Charlotte W.E. Leijten, Christina Grosserichter-Wagener, Trudy Voortman, Vincent V.W. Jaddoe, Menno C. Van Zelm, Jessica C. Kiefte-De Jong*

*Corresponding author for this work

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1 Citation (Scopus)
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Context: Adult obesity is associated with chronic low-grade inflammation and may give rise to future chronic disease. However, it is unclear whether adiposity-related inflammation is already apparent in childhood.

Objective: To study associations between child adiposity measures with circulating monocytes and naive and memory subsets in CD4, CD8, and γδT cell lineages.

Methods: Ten-year-old children (n = 890) from the Generation R Cohort underwent dual-energy x-ray absorptiometry and magnetic resonance imaging for body composition (body mass index [BMI], fat mass index [FMI], android-to-gynoid fat mass ratio, visceral fat index, liver fat fraction). Blood samples were taken for detailed immunophenotyping of leukocytes by 11-color flow cytometry.

Results: Several statistically significant associations were observed. A 1-SD increase in total FMI was associated with +8.4% (95% CI 2.0, 15.2) Vδ2+Vγ9+ and +7.4% (95% CI 2.4, 12.5) CD8+TEMRO cell numbers. A 1-SD increase in visceral fat index was associated with +10.7% (95% CI 3.3, 18.7) Vδ2+Vγ9+ and +8.3% (95% CI 2.6, 14.4) CD8+TEMRO cell numbers. Higher android-to-gynoid fat mass ratio was only associated with higher Vδ2+Vγ9+ T cells. Liver fat was associated with higher CD8+TEMRO cells but not with Vδ2+Vγ9+ T cells. Only liver fat was associated with lower Th17 cell numbers: a 1-SD increase was associated with -8.9% (95% CI -13.7, -3.7) Th17 cells. No associations for total CD8+, CD4+ T cells, or monocytes were observed. BMI was not associated with immune cells.

Conclusion: Higher Vδ2+Vγ9+ and CD8+TEMRO cell numbers in children with higher visceral fat index could reflect presence of adiposity-related inflammation in children with adiposity of a general population.

Original languageEnglish
Pages (from-to)E3923-E3935
JournalJournal of Clinical Endocrinology and Metabolism
Issue number10
Early online date15 Jun 2021
Publication statusPublished - 1 Oct 2021

Bibliographical note

Publisher Copyright:
© 2021 The Author(s) 2021. Published by Oxford University Press on behalf of the Endocrine Society. All rights reserved. For permissions, please e-mail: [email protected].

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  • EMC MM-04-54-08-A


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