Cleavage at a V(D)J recombination signal requires only RAG1 and RAG2 proteins and occurs in two steps

J. Fraser McBlane; Dik C. van Gent; Dale A. Ramsden; Charles Romeo; Christina A. Cuomo; Martin Gellert; Marjorie A. Oettinger

doi:10.1016/0092-8674(95)90116-7

Cleavage at a V(D)J recombination signal requires only RAG1 and RAG2 proteins and occurs in two steps

J. Fraser McBlane^*, Dik C. van Gent, Dale A. Ramsden, Charles Romeo, Christina A. Cuomo, Martin Gellert, Marjorie A. Oettinger

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

611 Citations (Scopus)

Abstract

Formation of double-strand breaks at recombination signal sequences is an early step in V(D)J recombination. Here we show that purified RAG1 and RAG2 proteins are sufficient to carry out this reaction. The cleavage reaction can be divided into two distinct steps. First, a nick is introduced at the 5′ end of the signal sequence. The other strand is then broken, resulting in a hairpin structure at the coding end and a blunt, 5′-phosphorylated signal end. The hairpin is made as a direct consequence of the cleavage mechanism. Nicking and hairpin formation each require the presence of a signal sequence and both RAG proteins.

Original language	English
Pages (from-to)	387-395
Number of pages	9
Journal	Cell
Volume	83
Issue number	3
DOIs	https://doi.org/10.1016/0092-8674(95)90116-7
Publication status	Published - 3 Nov 1995
Externally published	Yes

Access to Document

10.1016/0092-8674(95)90116-7

Cite this

@article{54c683b2da744e6cb91b8e63dbb95190,

title = "Cleavage at a V(D)J recombination signal requires only RAG1 and RAG2 proteins and occurs in two steps",

abstract = "Formation of double-strand breaks at recombination signal sequences is an early step in V(D)J recombination. Here we show that purified RAG1 and RAG2 proteins are sufficient to carry out this reaction. The cleavage reaction can be divided into two distinct steps. First, a nick is introduced at the 5′ end of the signal sequence. The other strand is then broken, resulting in a hairpin structure at the coding end and a blunt, 5′-phosphorylated signal end. The hairpin is made as a direct consequence of the cleavage mechanism. Nicking and hairpin formation each require the presence of a signal sequence and both RAG proteins.",

author = "McBlane, \{J. Fraser\} and \{van Gent\}, \{Dik C.\} and Ramsden, \{Dale A.\} and Charles Romeo and Cuomo, \{Christina A.\} and Martin Gellert and Oettinger, \{Marjorie A.\}",

year = "1995",

month = nov,

day = "3",

doi = "10.1016/0092-8674(95)90116-7",

language = "English",

volume = "83",

pages = "387--395",

journal = "Cell",

issn = "0092-8674",

publisher = "Elsevier",

number = "3",

}

TY - JOUR

T1 - Cleavage at a V(D)J recombination signal requires only RAG1 and RAG2 proteins and occurs in two steps

AU - McBlane, J. Fraser

AU - van Gent, Dik C.

AU - Ramsden, Dale A.

AU - Romeo, Charles

AU - Cuomo, Christina A.

AU - Gellert, Martin

AU - Oettinger, Marjorie A.

PY - 1995/11/3

Y1 - 1995/11/3

N2 - Formation of double-strand breaks at recombination signal sequences is an early step in V(D)J recombination. Here we show that purified RAG1 and RAG2 proteins are sufficient to carry out this reaction. The cleavage reaction can be divided into two distinct steps. First, a nick is introduced at the 5′ end of the signal sequence. The other strand is then broken, resulting in a hairpin structure at the coding end and a blunt, 5′-phosphorylated signal end. The hairpin is made as a direct consequence of the cleavage mechanism. Nicking and hairpin formation each require the presence of a signal sequence and both RAG proteins.

AB - Formation of double-strand breaks at recombination signal sequences is an early step in V(D)J recombination. Here we show that purified RAG1 and RAG2 proteins are sufficient to carry out this reaction. The cleavage reaction can be divided into two distinct steps. First, a nick is introduced at the 5′ end of the signal sequence. The other strand is then broken, resulting in a hairpin structure at the coding end and a blunt, 5′-phosphorylated signal end. The hairpin is made as a direct consequence of the cleavage mechanism. Nicking and hairpin formation each require the presence of a signal sequence and both RAG proteins.

UR - https://www.scopus.com/pages/publications/0028805853

U2 - 10.1016/0092-8674(95)90116-7

DO - 10.1016/0092-8674(95)90116-7

M3 - Article

C2 - 8521468

AN - SCOPUS:0028805853

SN - 0092-8674

VL - 83

SP - 387

EP - 395

JO - Cell

JF - Cell

IS - 3

ER -

Cleavage at a V(D)J recombination signal requires only RAG1 and RAG2 proteins and occurs in two steps

Abstract

Access to Document

Other files and links

Fingerprint

Cite this