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Clinical practice of indocyanine green fluorescence imaging in robotic liver surgery: a global expert survey

  • Noa L.E. Aegerter
  • , Christoph Kuemmerli
  • , Robotic Liver Surgery Expert Group
  • , Adrian T. Billeter
  • , Caroline Berchtold
  • , Felix Nickel
  • , Cristiano Guidetti
  • , Taiga Wakabayashi
  • , Iswanto Sucandy
  • , Brian K. Goh
  • , Mathieu D’Hondt
  • , Hugo Pinto Marques
  • , Janina Eden
  • , Philipp Dutkowski
  • , Jason Hawksworth
  • , Patrick Starlinger
  • , Beat P. Müller
  • , Philip C. Müller*
  • *Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Background: 

Indocyanine green (ICG) fluorescence imaging is increasingly incorporated into robotic liver resections (RLR), yet clinical practice regarding timing, dosage, and staining techniques is divergent. This international expert survey aimed to characterize current practices for ICG in RLR. 

Methods: 

Experts in RLR were invited to participate based on surgical volume (experience of ≥ 50 RLR and ≥ 30 annual RLR). A 74-item questionnaire was developed following a literature search and reviewed by a steering committee. The survey addressed indications, timing, dosage, imaging technology, benefits, limitations, training, and future directions of ICG use. Responses collected between September and October 2025 were analyzed. 

Results: 

Seventy experts from 19 countries completed the survey, corresponding to an 88% response rate. Centers performed a median of 180 annual liver resections, including 55 RLR. Most experts used ICG (96%) during RLR. Anatomical demarcation (91%), tumor localization (60%), and biliary anatomy assessment (60%) were the most frequent indications. 60% of experts use preoperative ICG, while intraoperative ICG is mainly administered for demarcation (67%) and biliary tract visualization (40%). Considerable heterogeneity exists in dosage, timing, and staining techniques, particularly in cirrhotic livers and for tumor localization. 53% of the experts had standard operating procedures, whereas 64% expressed the need for a higher degree of standardization. Reported benefits of ICG use included improved anatomical orientation, margin assessment, lesion detection, and support during complex resections. Perceived limitations included background fluorescence, tissue penetration and variable staining in diseased parenchyma. 80% anticipated improved outcomes with combined ICG and three-dimensional image-guidance. 

b

ICG fluorescence is widely used in RLR and is an important cornerstone for precision-guided robotic liver surgery. Standardized clinical practice guidelines, structured training, and technological improvements in imaging and navigation systems are claimed to optimize its clinical use.

Original languageEnglish
JournalSurgical Endoscopy
DOIs
Publication statusAccepted/In press - 9 Feb 2026

Bibliographical note

Publisher Copyright:
© The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2026.

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