Cluster analysis of genomic ETV6-RUNX1 (TEL-AML1) fusion sites in childhood acute lymphoblastic leukemia

H von Goessel; U Jacobs; S Semper; M Krumbholz; T Langer; T Keller; A Schrauder; Vincent van der Velden; Jacques Dongen; J Harbott; ER Panzer-Grumayer; M Schrappe; W Rascher; M Metzler

doi:10.1016/j.leukres.2008.11.001

Cluster analysis of genomic ETV6-RUNX1 (TEL-AML1) fusion sites in childhood acute lymphoblastic leukemia

H von Goessel, U Jacobs, S Semper, M Krumbholz, T Langer, T Keller, A Schrauder, Vincent van der Velden, Jacques Dongen, J Harbott, ER Panzer-Grumayer, M Schrappe, W Rascher, M Metzler

Immunology

Research output: Contribution to journal › Article › Academic › peer-review

18 Citations (Scopus)

Abstract

Fusion between ETV6 and RUNX1 defines the largest genetic subgroup in childhood ALL The genomic fusion site, unique to individual patients and specific for the malignant clone, represents an ideal molecular marker for quantification of minimal residual disease. Sequencing of DNA breakpoints has been difficult due to the extended size of the respective breakpoint cluster regions. We therefore evaluated a specially designed multiplex long-range PCR assay in 65 diagnostic bone marrow samples for its suitability in routine use. Resulting fusion sites and breakpoints derived from previous studies were subject to cluster analysis to identify potential sequence motifs involved in translocation initiation. (C) 2008 Elsevier Ltd. All rights reserved.

Original language	Undefined/Unknown
Pages (from-to)	1082-1088
Number of pages	7
Journal	Leukemia Research
Volume	33
Issue number	8
DOIs	https://doi.org/10.1016/j.leukres.2008.11.001
Publication status	Published - 2009

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10.1016/j.leukres.2008.11.001

http://hdl.handle.net/1765/24461

Cite this

von Goessel, H., Jacobs, U., Semper, S., Krumbholz, M., Langer, T., Keller, T., Schrauder, A., van der Velden, V., Dongen, J., Harbott, J., Panzer-Grumayer, ER., Schrappe, M., Rascher, W., & Metzler, M. (2009). Cluster analysis of genomic ETV6-RUNX1 (TEL-AML1) fusion sites in childhood acute lymphoblastic leukemia. Leukemia Research, 33(8), 1082-1088. https://doi.org/10.1016/j.leukres.2008.11.001

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title = "Cluster analysis of genomic ETV6-RUNX1 (TEL-AML1) fusion sites in childhood acute lymphoblastic leukemia",

abstract = "Fusion between ETV6 and RUNX1 defines the largest genetic subgroup in childhood ALL The genomic fusion site, unique to individual patients and specific for the malignant clone, represents an ideal molecular marker for quantification of minimal residual disease. Sequencing of DNA breakpoints has been difficult due to the extended size of the respective breakpoint cluster regions. We therefore evaluated a specially designed multiplex long-range PCR assay in 65 diagnostic bone marrow samples for its suitability in routine use. Resulting fusion sites and breakpoints derived from previous studies were subject to cluster analysis to identify potential sequence motifs involved in translocation initiation. (C) 2008 Elsevier Ltd. All rights reserved.",

author = "{von Goessel}, H and U Jacobs and S Semper and M Krumbholz and T Langer and T Keller and A Schrauder and {van der Velden}, Vincent and Jacques Dongen and J Harbott and ER Panzer-Grumayer and M Schrappe and W Rascher and M Metzler",

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von Goessel, H, Jacobs, U, Semper, S, Krumbholz, M, Langer, T, Keller, T, Schrauder, A, van der Velden, V, Dongen, J, Harbott, J, Panzer-Grumayer, ER, Schrappe, M, Rascher, W & Metzler, M 2009, 'Cluster analysis of genomic ETV6-RUNX1 (TEL-AML1) fusion sites in childhood acute lymphoblastic leukemia', Leukemia Research, vol. 33, no. 8, pp. 1082-1088. https://doi.org/10.1016/j.leukres.2008.11.001

TY - JOUR

T1 - Cluster analysis of genomic ETV6-RUNX1 (TEL-AML1) fusion sites in childhood acute lymphoblastic leukemia

AU - von Goessel, H

AU - Jacobs, U

AU - Semper, S

AU - Krumbholz, M

AU - Langer, T

AU - Keller, T

AU - Schrauder, A

AU - van der Velden, Vincent

AU - Dongen, Jacques

AU - Harbott, J

AU - Panzer-Grumayer, ER

AU - Schrappe, M

AU - Rascher, W

AU - Metzler, M

PY - 2009

Y1 - 2009

N2 - Fusion between ETV6 and RUNX1 defines the largest genetic subgroup in childhood ALL The genomic fusion site, unique to individual patients and specific for the malignant clone, represents an ideal molecular marker for quantification of minimal residual disease. Sequencing of DNA breakpoints has been difficult due to the extended size of the respective breakpoint cluster regions. We therefore evaluated a specially designed multiplex long-range PCR assay in 65 diagnostic bone marrow samples for its suitability in routine use. Resulting fusion sites and breakpoints derived from previous studies were subject to cluster analysis to identify potential sequence motifs involved in translocation initiation. (C) 2008 Elsevier Ltd. All rights reserved.

AB - Fusion between ETV6 and RUNX1 defines the largest genetic subgroup in childhood ALL The genomic fusion site, unique to individual patients and specific for the malignant clone, represents an ideal molecular marker for quantification of minimal residual disease. Sequencing of DNA breakpoints has been difficult due to the extended size of the respective breakpoint cluster regions. We therefore evaluated a specially designed multiplex long-range PCR assay in 65 diagnostic bone marrow samples for its suitability in routine use. Resulting fusion sites and breakpoints derived from previous studies were subject to cluster analysis to identify potential sequence motifs involved in translocation initiation. (C) 2008 Elsevier Ltd. All rights reserved.

U2 - 10.1016/j.leukres.2008.11.001

DO - 10.1016/j.leukres.2008.11.001

M3 - Article

SN - 0145-2126

VL - 33

SP - 1082

EP - 1088

JO - Leukemia Research

JF - Leukemia Research

IS - 8

ER -