Combined Analysis of Transcriptome and T-Cell Receptor Alpha and Beta (TRA /TRB ) Repertoire in Paucicellular Samples at the Single-Cell Level

Nicolle H R Litjens, Anton W Langerak, Zakia Azmani, Xander den Dekker, Michiel G H Betjes, Rutger W W Brouwer, Wilfred F J van IJcken*

*Corresponding author for this work

Research output: Chapter/Conference proceedingChapterAcademic

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Abstract

With the advent of next-generation sequencing (NGS) methodologies, the total repertoires of B and T cells can be disclosed in much more detail than ever before. Even though many of these strategies do provide in-depth and high-resolution information of the immunoglobulin (IG) and/or T-cell receptor (TR) repertoire, one clear disadvantage is that the IG/TR profiles cannot be connected to individual cells. Single-cell technologies do allow to study the IG/TR repertoire at the individual cell level. This is especially relevant in cell samples in which much heterogeneity of the cell population is expected. By combining the IG/TR repertoire with transcriptome data, the reactivity of the B or T cell can be associated with activation or maturation stages. An additional advantage of such single-cell technologies is that the combination of both IG and both TR chains can be studied on a per cell basis, which better reflects the antigen receptor reactivity of cells. Here we present the ICELL8 single-cell method for the parallel analysis of the TR repertoire and transcriptome, which is especially useful in samples that contain relatively few cells.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
Subtitle of host publicationMethods and Protocols
PublisherHumana Press
Pages231-259
Number of pages29
Volume2453
Edition1
ISBN (Electronic)978-1-0716-2115-8
ISBN (Print)978-1-0716-2114-1
DOIs
Publication statusPublished - 28 May 2022

Publication series

SeriesMethods in Molecular Biology
ISSN1064-3745

Bibliographical note

Funding Information:
We would like to thank Maaike de Bie, Amy van der List, and Mariska Klepper for technical assistance.

Publisher Copyright:
© 2022, The Author(s).

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