Combined TCRG and TCRA TREC analysis reveals increased peripheral T-lymphocyte but constant intra-thymic proliferative history upon ageing

Kim Weerd, Wim Dik, Benjamin Schrijver, Ad Bogers, A.P.W.M. Maat, Francien Nederveen, P.M. van Hagen, Jacques Dongen, Ton Langerak, Frank Staal

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Abstract

T-cell receptor (TCR) repertoire diversity, thymic output, clonal size and peripheral T-lymphocyte numbers largely depend on intra-thymic and post-thymic T-lymphocyte proliferation. However, quantitative insight into thymocyte and T-lymphocyte proliferation is still lacking. We developed a new TCRG-based TCR excision circle (TREC) assay, the V gamma-J gamma TREC assay, which we used together with an adjusted delta REC-psi J alpha TREC assay to quantify the proliferative history of human thymocyte and T-lymphocyte subpopulations from children and adults. This revealed that thymocytes undergo similar to 6-8 intra-thymic cell divisions from the double negative (DN) 3 developmental stage onwards, which appeared independent of age. Thus thymocyte proliferation after the DN3 developmental stages is stable and therefore not contributing to the reduced thymic output upon ageing. Cord blood naive T lymphocytes had already undergone similar to 2-3 post-thymic cell divisions, which increased to similar to 6-7 cell divisions in naive T lymphocytes of middle-aged adults, indicating the importance of homeostatic naive T-lymphocyte proliferation from a young age onwards in the maintenance of peripheral T-lymphocyte numbers. In conclusion, our data provide quantitative insight into the proliferative history of thymocyte and T-lymphocyte subpopulations and alterations herein upon ageing. This novel TREC assay approach could prove valuable in immune status monitoring in a variety of conditions. (C) 2012 Elsevier Ltd. All rights reserved.
Original languageUndefined/Unknown
Pages (from-to)302-312
Number of pages11
JournalMolecular Immunology
Volume53
Issue number3
DOIs
Publication statusPublished - 2013

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