Comparative Analysis of SARS-CoV-2 Antigenicity across Assays and in Human and Animal Model Sera

Barbara Mühlemann; Samuel H. Wilks; Lauren Baracco; Meriem Bekliz; Juan Manuel Carreño; Victor M. Corman; Meredith E. Davis-Gardner; Wanwisa Dejnirattisai; Michael S. Diamond; Daniel C. Douek; Christian Drosten; Isabella Eckerle; Venkata Viswanadh Edara; Madison Ellis; Ron A M Fouchier; Matthew Frieman; Sucheta Godbole; Bart Haagmans; Peter J. Halfmann; Amy R. Henry; Terry Jones; Leah C. Katzelnick; Yoshihiro Kawaoka; Janine Kimpel; Florian Krammer; Lilin Lai; Chang Liu; Sabrina Lusvarghi; Benjamin Meyer; Juthathip Mongkolsapaya; David C. Montefiori; Anna Mykytyn; Antonia Netzl; Simon Pollett; Annika Rössler; Gavin R Screaton; Xiaoying Shen; Alex Sigal; Viviana Simon; Rahul Subramanian; Piyada Supasa; Mehul Suthar; Sina Türeli; Wei Wang; Carol D Weiss; Derek J Smith

doi:10.1101/2023.09.27.559689

Comparative Analysis of SARS-CoV-2 Antigenicity across Assays and in Human and Animal Model Sera

Barbara Mühlemann
, Samuel H. Wilks
, Lauren Baracco
, Meriem Bekliz
, Juan Manuel Carreño
, Victor M. Corman
, Meredith E. Davis-Gardner
, Wanwisa Dejnirattisai
, Michael S. Diamond
, Daniel C. Douek
, Christian Drosten
, Isabella Eckerle
, Venkata Viswanadh Edara
, Madison Ellis
, Ron A M Fouchier
, Matthew Frieman
, Sucheta Godbole
, Bart Haagmans
, Peter J. Halfmann
, Amy R. Henry

Terry Jones, Leah C. Katzelnick, Yoshihiro Kawaoka, Janine Kimpel, Florian Krammer, Lilin Lai, Chang Liu, Sabrina Lusvarghi, Benjamin Meyer, Juthathip Mongkolsapaya, David C. Montefiori, Anna Mykytyn, Antonia Netzl, Simon Pollett, Annika Rössler, Gavin R Screaton, Xiaoying Shen, Alex Sigal, Viviana Simon, Rahul Subramanian, Piyada Supasa, Mehul Suthar, Sina Türeli, Wei Wang, Carol D Weiss, Derek J Smith

Virology

Research output: Contribution to journal › Article › Academic

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Abstract

The antigenic evolution of SARS-CoV-2 requires ongoing monitoring to judge the immune escape of newly arising variants. A surveillance system necessitates an understanding of differences in neutralization titers measured in different assays and using human and animal sera. We compared 18 datasets generated using human, hamster, and mouse sera, and six different neutralization assays. Titer magnitude was lowest in human, intermediate in hamster, and highest in mouse sera. Fold change, immunodominance patterns and antigenic maps were similar among sera. Most assays yielded similar results, except for differences in fold change in cytopathic effect assays. Not enough data was available for conclusively judging mouse sera, but hamster sera were a consistent surrogate for human first-infection sera.

Original language	English
Journal	bioRxiv : the preprint server for biology
DOIs	https://doi.org/10.1101/2023.09.27.559689
Publication status	Published - 27 Sept 2023

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2023.09.27.559689v1.fullAccepted author manuscript, 1.87 MBLicence: CC BY-NC-ND

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Mühlemann, B., Wilks, S. H., Baracco, L., Bekliz, M., Carreño, J. M., Corman, V. M., Davis-Gardner, M. E., Dejnirattisai, W., Diamond, M. S., Douek, D. C., Drosten, C., Eckerle, I., Edara, V. V., Ellis, M., Fouchier, R. A. M., Frieman, M., Godbole, S., Haagmans, B., Halfmann, P. J., ... Smith, D. J. (2023). Comparative Analysis of SARS-CoV-2 Antigenicity across Assays and in Human and Animal Model Sera. bioRxiv : the preprint server for biology. https://doi.org/10.1101/2023.09.27.559689

@article{05b9570755554b38b3a05b71b17a7fa2,

title = "Comparative Analysis of SARS-CoV-2 Antigenicity across Assays and in Human and Animal Model Sera",

abstract = "The antigenic evolution of SARS-CoV-2 requires ongoing monitoring to judge the immune escape of newly arising variants. A surveillance system necessitates an understanding of differences in neutralization titers measured in different assays and using human and animal sera. We compared 18 datasets generated using human, hamster, and mouse sera, and six different neutralization assays. Titer magnitude was lowest in human, intermediate in hamster, and highest in mouse sera. Fold change, immunodominance patterns and antigenic maps were similar among sera. Most assays yielded similar results, except for differences in fold change in cytopathic effect assays. Not enough data was available for conclusively judging mouse sera, but hamster sera were a consistent surrogate for human first-infection sera.",

author = "Barbara M{\"u}hlemann and Wilks, \{Samuel H.\} and Lauren Baracco and Meriem Bekliz and Carre{\~n}o, \{Juan Manuel\} and Corman, \{Victor M.\} and Davis-Gardner, \{Meredith E.\} and Wanwisa Dejnirattisai and Diamond, \{Michael S.\} and Douek, \{Daniel C.\} and Christian Drosten and Isabella Eckerle and Edara, \{Venkata Viswanadh\} and Madison Ellis and Fouchier, \{Ron A M\} and Matthew Frieman and Sucheta Godbole and Bart Haagmans and Halfmann, \{Peter J.\} and Henry, \{Amy R.\} and Terry Jones and Katzelnick, \{Leah C.\} and Yoshihiro Kawaoka and Janine Kimpel and Florian Krammer and Lilin Lai and Chang Liu and Sabrina Lusvarghi and Benjamin Meyer and Juthathip Mongkolsapaya and Montefiori, \{David C.\} and Anna Mykytyn and Antonia Netzl and Simon Pollett and Annika R{\"o}ssler and Screaton, \{Gavin R\} and Xiaoying Shen and Alex Sigal and Viviana Simon and Rahul Subramanian and Piyada Supasa and Mehul Suthar and Sina T{\"u}reli and Wei Wang and Weiss, \{Carol D\} and Smith, \{Derek J\}",

year = "2023",

month = sep,

day = "27",

doi = "10.1101/2023.09.27.559689",

language = "English",

}

Mühlemann, B, Wilks, SH, Baracco, L, Bekliz, M, Carreño, JM, Corman, VM, Davis-Gardner, ME, Dejnirattisai, W, Diamond, MS, Douek, DC, Drosten, C, Eckerle, I, Edara, VV, Ellis, M, Fouchier, RAM, Frieman, M, Godbole, S, Haagmans, B, Halfmann, PJ, Henry, AR, Jones, T, Katzelnick, LC, Kawaoka, Y, Kimpel, J, Krammer, F, Lai, L, Liu, C, Lusvarghi, S, Meyer, B, Mongkolsapaya, J, Montefiori, DC, Mykytyn, A, Netzl, A, Pollett, S, Rössler, A, Screaton, GR, Shen, X, Sigal, A, Simon, V, Subramanian, R, Supasa, P, Suthar, M, Türeli, S, Wang, W, Weiss, CD & Smith, DJ 2023, 'Comparative Analysis of SARS-CoV-2 Antigenicity across Assays and in Human and Animal Model Sera', bioRxiv : the preprint server for biology. https://doi.org/10.1101/2023.09.27.559689

TY - JOUR

T1 - Comparative Analysis of SARS-CoV-2 Antigenicity across Assays and in Human and Animal Model Sera

AU - Mühlemann, Barbara

AU - Wilks, Samuel H.

AU - Baracco, Lauren

AU - Bekliz, Meriem

AU - Carreño, Juan Manuel

AU - Corman, Victor M.

AU - Davis-Gardner, Meredith E.

AU - Dejnirattisai, Wanwisa

AU - Diamond, Michael S.

AU - Douek, Daniel C.

AU - Drosten, Christian

AU - Eckerle, Isabella

AU - Edara, Venkata Viswanadh

AU - Ellis, Madison

AU - Fouchier, Ron A M

AU - Frieman, Matthew

AU - Godbole, Sucheta

AU - Haagmans, Bart

AU - Halfmann, Peter J.

AU - Henry, Amy R.

AU - Jones, Terry

AU - Katzelnick, Leah C.

AU - Kawaoka, Yoshihiro

AU - Kimpel, Janine

AU - Krammer, Florian

AU - Lai, Lilin

AU - Liu, Chang

AU - Lusvarghi, Sabrina

AU - Meyer, Benjamin

AU - Mongkolsapaya, Juthathip

AU - Montefiori, David C.

AU - Mykytyn, Anna

AU - Netzl, Antonia

AU - Pollett, Simon

AU - Rössler, Annika

AU - Screaton, Gavin R

AU - Shen, Xiaoying

AU - Sigal, Alex

AU - Simon, Viviana

AU - Subramanian, Rahul

AU - Supasa, Piyada

AU - Suthar, Mehul

AU - Türeli, Sina

AU - Wang, Wei

AU - Weiss, Carol D

AU - Smith, Derek J

PY - 2023/9/27

Y1 - 2023/9/27

N2 - The antigenic evolution of SARS-CoV-2 requires ongoing monitoring to judge the immune escape of newly arising variants. A surveillance system necessitates an understanding of differences in neutralization titers measured in different assays and using human and animal sera. We compared 18 datasets generated using human, hamster, and mouse sera, and six different neutralization assays. Titer magnitude was lowest in human, intermediate in hamster, and highest in mouse sera. Fold change, immunodominance patterns and antigenic maps were similar among sera. Most assays yielded similar results, except for differences in fold change in cytopathic effect assays. Not enough data was available for conclusively judging mouse sera, but hamster sera were a consistent surrogate for human first-infection sera.

AB - The antigenic evolution of SARS-CoV-2 requires ongoing monitoring to judge the immune escape of newly arising variants. A surveillance system necessitates an understanding of differences in neutralization titers measured in different assays and using human and animal sera. We compared 18 datasets generated using human, hamster, and mouse sera, and six different neutralization assays. Titer magnitude was lowest in human, intermediate in hamster, and highest in mouse sera. Fold change, immunodominance patterns and antigenic maps were similar among sera. Most assays yielded similar results, except for differences in fold change in cytopathic effect assays. Not enough data was available for conclusively judging mouse sera, but hamster sera were a consistent surrogate for human first-infection sera.

U2 - 10.1101/2023.09.27.559689

DO - 10.1101/2023.09.27.559689

M3 - Article

C2 - 37808679

SN - 2692-8205

JO - bioRxiv : the preprint server for biology

JF - bioRxiv : the preprint server for biology

ER -

Comparative Analysis of SARS-CoV-2 Antigenicity across Assays and in Human and Animal Model Sera

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