Comparison of laser speckle contrast imaging with laser Doppler perfusion imaging for tissue perfusion measurement

Goksel Guven*, Annemieke Dijkstra, Tjallingius Martijn Kuijper, Nicole Trommel, Margriet Elisabeth van Baar, Arzu Topeli, Can Ince, Cornelis Hendrik van der Vlies

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

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Abstract

Objective: Laser-based tissue perfusion monitoring techniques have been increasingly used in animal and human research to assess blood flow. However, these techniques use arbitrary units, and knowledge about their comparability is scarce. This study aimed to model the relationship between laser speckle contrast imaging (LSCI) and laser Doppler perfusion imaging (LDPI), for measuring tissue perfusion over a wide range of blood flux values. Methods: Fifteen healthy volunteers (53% female, median age 29 [IQR 22–40] years) were enrolled in this study. We performed iontophoresis with sodium nitroprusside on the forearm to induce regional vasodilation to increase skin blood flux. Besides, a stepwise vascular occlusion was applied on the contralateral upper arm to reduce blood flux. Both techniques were compared using a linear mixed model analysis. Results: Baseline blood flux values measured by LSCI were 33 ± 6.5 arbitrary unit (AU) (Coefficient of variation [CV] = 20%) and by LDPI 60 ± 11.5 AU (CV = 19%). At the end of the iontophoresis protocol, the regional blood flux increased to 724 ± 412% and 259 ± 87% of baseline measured by LDPI and LSCI, respectively. On the other hand, during the stepwise vascular occlusion test, the blood flux reduced to 212 ± 40% and 412 ± 177% of its baseline at LDPI and LSCI, respectively. A strong correlation was found between the LSCI and LDPI instruments at increased blood flux with respect to baseline skin blood flux; however, the correlation was weak at reduced blood flux with respect to baseline. Discussion: LSCI and LDPI instruments are highly linear for blood flux higher than baseline skin blood flux; however, the correlation decreased for blood flux lower than baseline. This study's findings could be a basis for using LSCI in specific patient populations, such as burn care.

Original languageEnglish
Article numbere12795
JournalMicrocirculation
DOIs
Publication statusE-pub ahead of print - 15 Dec 2022

Bibliographical note

FUNDING INFORMATION
The collaboration project is co-funded by the PPP Allowance to stimulate public-private partnerships made available by Health~Holland,
Top Sector Life Sciences & Health, as well as by a contribution from
Dutch Burns Foundation.


Publisher Copyright:
© 2022 The Authors. Microcirculation published by John Wiley & Sons Ltd.

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